Zebrafish Psmd7 Antibody / 26S proteasome non-ATPase regulatory subunit 7 (RZ1295)

Description

Psmd7 (Proteasome 26S subunit, non-ATPase 7) is an important component of the 19S regulatory particle of the 26S proteasome, which is responsible for the ATP-dependent degradation of ubiquitinated proteins. As part of the proteasome complex, Psmd7 contributes to the recognition, unfolding, and translocation of substrates into the 20S catalytic core where they are degraded. This process is essential for cellular homeostasis, regulation of the cell cycle, and the maintenance of protein integrity by ensuring the timely degradation of damaged, misfolded, or excess proteins.

In zebrafish, Psmd7 is the ortholog of the human PSMD7 gene, sharing a high degree of sequence similarity and functional conservation. The zebrafish and human versions of Psmd7 contain similar domains that allow interaction with other proteasomal subunits, enabling the proper assembly and function of the 26S proteasome complex. This functional conservation underscores the relevance of zebrafish as a model organism for studying proteasomal processes and their role in human health.

Psmd7 in zebrafish may have isoforms resulting from alternative splicing, although specific isoforms may not be fully characterized. These isoforms could play roles in different tissues or developmental stages, contributing to the fine-tuning of proteasomal activity. Isoform variation could also provide differential regulation of proteasome function in response to environmental stress, cellular signaling, or developmental needs.

In zebrafish, Psmd7 is expressed in a variety of tissues, especially those with high protein turnover, such as the brain, liver, and kidney. This expression pattern reflects the protein's essential role in controlling protein degradation, supporting cellular functions such as stress response, apoptosis, and differentiation.

Given the high conservation between zebrafish Psmd7 and human PSMD7, the zebrafish model is valuable for studying proteasomal dysfunctions that contribute to diseases in humans, such as cancer, neurodegenerative disorders, and immune dysfunctions. Disruption in PSMD7 has been linked to altered proteasomal activity, which can lead to the accumulation of damaged proteins and cellular stress. The zebrafish model can be used to explore potential therapeutic approaches aimed at modulating proteasomal function and preventing the pathological effects associated with these diseases.

Application Notes

Optimal dilution of the Zebrafish Psmd7 antibody should be determined by the researcher.

Immunogen

E. coli-derived zebrafish Psmd7 recombinant protein (amino acids M1-N274) was used as the immunogen for the Zebrafish Psmd7 antibody.

Storage

After reconstitution, the Zebrafish Psmd7 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.