Zebrafish Prpf6 Antibody / Pre-mRNA processing factor 6 (RZ1283)

Description

Prpf6 (Pre-mRNA processing factor 6) is an essential component of the spliceosome, the complex responsible for the excision of introns from pre-messenger RNA during the gene expression process. It plays a key role in the assembly and stabilization of the U4, U5, and U6 small nuclear ribonucleoproteins into the mature tri-snRNP complex. Prpf6 acts as a bridging factor within the spliceosome and is critical for ensuring efficient and accurate pre-mRNA splicing.

In zebrafish, Prpf6 is an ortholog of the human PRPF6 gene. The zebrafish and human proteins share a high degree of conservation, particularly in domains involved in protein-protein interactions within the spliceosome. This evolutionary conservation supports the use of zebrafish as a reliable model for studying the functional roles of spliceosomal components and for understanding the molecular basis of splicing-related diseases in humans.

Zebrafish Prpf6 may have isoforms generated through alternative splicing. These isoforms may differ in their regulatory features or expression patterns, enabling context-specific or tissue-specific control of splicing events during development or in response to physiological signals.

During embryonic development in zebrafish, Prpf6 is broadly expressed in actively proliferating and transcriptionally active tissues, such as the central nervous system, eye, and somites. Its function is critical for cell viability, as defects in pre-mRNA splicing can result in misexpression of genes required for cell cycle progression, differentiation, and organogenesis.

In humans, mutations in PRPF6 have been linked to retinitis pigmentosa and other splicing-associated disorders. Because of the conserved function and expression of Prpf6 in zebrafish, it serves as a valuable model for investigating the cellular consequences of spliceosomal dysfunction and for testing therapeutic approaches targeting RNA processing defects.

Application Notes

Optimal dilution of the Zebrafish Prpf6 antibody should be determined by the researcher.

Immunogen

E. coli-derived zebrafish Prpf6 recombinant protein (amino acids Q99-K618) was used as the immunogen for the Zebrafish Prpf6 antibody.

Storage

After reconstitution, the Zebrafish Prpf6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.