Zebrafish PIWIL1 Antibody / Piwi-like Protein 1 Antibody (RZ1395)

Zebrafish PIWIL1 Antibody IHC. Immunohistochemistry staining of paraffin-embedded zebrafish brain tissue using Zebrafish PIWIL1 Antibody / Piwi-like Protein 1 Antibody demonstrates cytoplasmic and perinuclear HRP-DAB brown staining within discrete cellular populations. The staining pattern is consistent with expression of PIWIL1 (Ziwi), a germline-associated PIWI family protein involved in piRNA-mediated gene silencing, transposon suppression, genome stability, and stem cell maintenance. PIWIL1 is a key regulator of germ cell development and reproductive biology and serves as an important marker for studies of germline specification and developmental genetics in zebrafish. HIER was performed using EDTA buffer. Tissue sections were blocked with 10% goat serum and incubated with primary antibody at 2 µg/ml overnight at 4°C prior to detection with HRP-conjugated secondary antibody and DAB chromogen.

Zebrafish PIWIL1 Antibody Ovary IHC. Immunohistochemistry staining of paraffin-embedded zebrafish ovary tissue using Zebrafish PIWIL1 Antibody / Piwi-like Protein 1 Antibody demonstrates cytoplasmic HRP-DAB brown staining within ovarian germ cell-associated compartments. The staining pattern is consistent with expression of PIWIL1 (Ziwi), a germline-specific PIWI family protein that regulates piRNA-mediated gene silencing, transposon suppression, genome integrity, and germ cell maintenance. PIWIL1 is an established marker of germline development and reproductive biology in zebrafish and plays a critical role in oogenesis and long-term preservation of germ cell populations. HIER was performed using EDTA buffer. Tissue sections were blocked with 10% goat serum and incubated with primary antibody at 2 µg/ml overnight at 4°C prior to detection with HRP-conjugated secondary antibody and DAB chromogen.

Zebrafish PIWIL1 Antibody Eye IHC. Immunohistochemistry staining of paraffin-embedded zebrafish eye tissue using Zebrafish PIWIL1 Antibody / Piwi-like Protein 1 Antibody demonstrates prominent HRP-DAB brown staining within retinal-associated cellular layers. The staining pattern is consistent with expression of PIWIL1 (Ziwi), a member of the PIWI family of Argonaute proteins that functions in piRNA-mediated gene regulation, transposon suppression, genome stability, and germline maintenance. In zebrafish, PIWIL1 is best known for its role in germ cell development, although PIWI-pathway proteins have also been implicated in broader developmental and stem cell regulatory processes. HIER was performed using EDTA buffer. Tissue sections were blocked with 10% goat serum and incubated with primary antibody at 2 µg/ml overnight at 4°C prior to detection with HRP-conjugated secondary antibody and DAB chromogen.

Zebrafish PIWIL1 Antibody WB. Western blot analysis of whole female zebrafish tissue lysate using Zebrafish PIWIL1 Antibody / Piwi-like Protein 1 Antibody demonstrates a prominent immunoreactive band at approximately 97 kDa. PIWIL1 (Ziwi) is a member of the PIWI subfamily of Argonaute proteins and is a key regulator of germ cell development, piRNA-mediated gene silencing, transposon suppression, and genome stability in zebrafish. Expression of PIWIL1 is closely associated with germline cell populations and reproductive tissues, making it an important marker for studies of developmental and reproductive biology. Thirty micrograms of protein lysate were resolved under reducing conditions on a 10% SDS-PAGE gel and transferred to nitrocellulose prior to immunodetection. The observed band supports detection of PIWIL1 in zebrafish tissue lysates by western blot analysis. Predicted molecular weight: ~97 kDa.