Zebrafish P0 Antibody / MPZ Antibody (RZ1415)

Zebrafish P0 Antibody Brain IHC. Immunohistochemical analysis of paraffin-embedded zebrafish brain tissue using Zebrafish P0 Antibody / MPZ Antibody demonstrates prominent HRP-DAB brown staining within neural tissue structures and fiber-rich regions of the brain. The staining pattern is consistent with expression of P0 (Myelin Protein Zero), a major structural component of myelin that is essential for myelin sheath formation, axonal insulation, and maintenance of normal nerve conduction. Positive immunoreactivity highlights regions associated with myelinated neural pathways and supports the role of MPZ in nervous system development and myelin organization. Heat-induced antigen retrieval was performed in EDTA buffer. Tissue sections were blocked with 10% goat serum and incubated with P0/MPZ antibody (2 ug/ml) overnight at 4°C, followed by Peroxidase Conjugated Goat Anti-rabbit IgG and DAB chromogen detection.

Zebrafish P0 Antibody Brain IHC. Immunohistochemical analysis of paraffin-embedded zebrafish brain tissue using Zebrafish P0 Antibody / MPZ Antibody demonstrates strong HRP-DAB brown staining within fiber-rich neural regions adjacent to densely cellular neuronal layers. The staining pattern is consistent with expression of P0 (Myelin Protein Zero), the principal structural protein of peripheral myelin that mediates myelin membrane adhesion and supports normal nerve conduction. Positive immunoreactivity is associated with myelinated neural processes and regions involved in nervous system organization, highlighting the role of MPZ in myelin sheath formation and maintenance. Heat-induced antigen retrieval was performed in EDTA buffer. Tissue sections were blocked with 10% goat serum and incubated with P0/MPZ antibody (2 ug/ml) overnight at 4°C, followed by Peroxidase Conjugated Goat Anti-rabbit IgG and DAB chromogen detection.

Zebrafish P0 Antibody Multi-Tissue WB. Western blot analysis of 1) zebrafish head tissue lysate, 2) whole female zebrafish lysate, and 3) whole male zebrafish lysate using Zebrafish P0 Antibody / MPZ Antibody. A prominent immunoreactive band is detected at approximately 22 kDa in all samples, consistent with the expected molecular weight of P0 (Myelin Protein Zero/MPZ). P0 is the major structural protein of peripheral myelin and plays a critical role in myelin sheath compaction, axonal insulation, and maintenance of normal nerve conduction. The observed expression in zebrafish tissues supports the importance of MPZ in nervous system development and myelin organization. Minor lower-molecular-weight bands observed in some samples may represent processed forms, differential glycosylation states, or protein degradation products commonly detected for myelin-associated proteins. Electrophoresis was performed on a 12% SDS-PAGE gel under reducing conditions. Predicted molecular weight: ~22 kDa. Observed molecular weight: ~22 kDa.

Zebrafish P0 Antibody Adult Brain IF. Immunofluorescence analysis of adult zebrafish brain tissue using Zebrafish P0 Antibody. P0, encoded by the zebrafish mpz gene, was detected in a paraffin-embedded brain section following heat induced antigen retrieval in pH 8.0 EDTA buffer. The tissue was incubated overnight at 4°C with primary antibody (5 ug/mL), followed by Fluoro488-conjugated secondary antibody. DAPI (blue) was used as a nuclear counterstain. Green fluorescence highlights P0-positive structures within the adult zebrafish brain, supporting the use of Zebrafish P0 Antibody for investigating myelin protein zero expression, peripheral myelin biology and nervous system development.

Zebrafish P0 Antibody Myelinated Fiber IF. P0, encoded by the zebrafish mpz gene, was detected in a paraffin-embedded section of adult zebrafish brain tissue. Heat induced antigen retrieval was performed in pH 8.0 EDTA buffer prior to overnight incubation with Zebrafish P0 Antibody (5 ug/mL) at 4°C, followed by Fluoro488-conjugated secondary antibody. DAPI (blue) was used as a nuclear counterstain. Green fluorescence highlights P0-positive myelinated fiber tracts surrounding the brain parenchyma, consistent with the expected distribution of myelin protein zero. These results support the use of Zebrafish P0 Antibody for investigating mpz, peripheral myelin formation and nervous system development.