Zebrafish Nkx2.2a Antibody / Nkx2.2 (RZ1250)

Description

Nkx2.2a is a homeobox transcription factor that plays a critical role in the development of the central nervous system and pancreas in zebrafish. It belongs to the NK class of homeodomain proteins and functions by regulating gene expression involved in cell fate determination and patterning during embryogenesis.

Zebrafish Nkx2.2a is one of two paralogs, the other being Nkx2.2b, resulting from a genome duplication event in teleosts. Nkx2.2a is considered a functional ortholog of the human NKX2 dash 2 gene. Both proteins are highly conserved and share similar developmental roles, particularly in the specification of ventral neural progenitors and in endocrine pancreas formation.

In zebrafish embryos, Nkx2.2a is expressed in the ventral neural tube, including the regions that give rise to motor neurons, oligodendrocyte precursors, and serotonergic neurons. It acts downstream of sonic hedgehog signaling and is essential for defining the identity of specific neuronal subtypes. Nkx2.2a also contributes to the early development of the pancreas, where it helps regulate differentiation of insulin producing beta cells and other endocrine cell types.

Loss of Nkx2.2a function in zebrafish leads to defects in neuronal patterning and impaired endocrine pancreas development, mirroring the phenotypes observed in mammalian systems. Due to its conservation with the human NKX2 dash 2 gene and its involvement in key developmental processes, zebrafish Nkx2.2a is widely used as a model to study neurogenesis, pancreas biology, and transcriptional regulation in vertebrates.

Application Notes

Optimal dilution of the Zebrafish Nkx2.2a antibody should be determined by the researcher.

Immunogen

E. coli-derived zebrafish Nkx2.2a recombinant protein (amino acids M1-W269) was used as the immunogen for the Zebrafish Nkx2.2a antibody.

Storage

After reconstitution, the Zebrafish Nkx2.2a antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.