Zebrafish NKX2.1 Antibody / Thyroid Transcription Factor 1 Antibody (RZ1473)

Zebrafish NKX2.1 Antibody / Thyroid Transcription Factor 1 Antibody Zebrafish Spinal Cord IHC. Immunohistochemical staining of paraffin-embedded zebrafish spinal cord tissue using Zebrafish NKX2.1 Antibody / Thyroid Transcription Factor 1 Antibody. NKX2.1 expression is detected predominantly within the nuclei of spinal cord cells, consistent with its function as a homeobox transcription factor regulating neuronal specification, central nervous system development, and transcriptional control during vertebrate embryogenesis. Heat-induced epitope retrieval was performed using EDTA buffer prior to immunostaining with DAB chromogenic detection.

Zebrafish NKX2.1 Antibody / Thyroid Transcription Factor 1 Antibody Zebrafish Head, Female and Male WB. Western blot analysis of zebrafish head, whole female, and whole male tissue lysates using Zebrafish NKX2.1 Antibody / Thyroid Transcription Factor 1 Antibody. A distinct immunoreactive band is detected at approximately 42 kDa in all three samples, consistent with expression of NKX2.1. The observed molecular weight is close to the predicted size of approximately 38 kDa, supporting specific detection of the target protein. Proteins were separated under reducing conditions, transferred to a nitrocellulose membrane, and detected following incubation with the primary antibody (0.5 ug/ml) and an HRP-conjugated goat anti-rabbit secondary antibody using ECL detection.