Zebrafish Ndr1 Antibody / Nodal-related 1 (RZ1247)

Description

Nodal related 1, also known as Squint and Ndr1, is a member of the transforming growth factor beta superfamily and plays a key role in early embryonic development in zebrafish. It is involved in the establishment of the body axis, mesoderm and endoderm formation, and the specification of left right asymmetry. Nodal related 1 functions as a secreted signaling molecule that activates intracellular pathways through binding to type one and type two serine threonine kinase receptors.

Zebrafish Nodal related 1 is considered a functional homolog of human NODAL, although it is not a direct one to one ortholog due to lineage specific gene duplications in teleost fish. However, both proteins are part of the same signaling pathway and perform highly conserved roles in vertebrate embryogenesis. Like human NODAL, zebrafish Nodal related 1 signals through the SMAD family of transcription factors and is regulated by feedback mechanisms involving antagonists such as Lefty and Cerberus.

Expression of Nodal related 1 begins during the blastula stage and becomes concentrated in the embryonic margin, where it helps induce and pattern the mesendoderm. Mutations or knockdown of this gene result in severe defects in gastrulation, germ layer formation, and organ development. Due to its essential role in vertebrate development and strong functional conservation, zebrafish Nodal related 1 is widely studied in developmental biology, cell signaling, and disease modeling.

Application Notes

Optimal dilution of the Zebrafish Ndr1 antibody should be determined by the researcher.

Immunogen

E. coli-derived zebrafish Ndr1 recombinant protein (amino acids Q28-D296) was used as the immunogen for the Zebrafish Ndr1 antibody.

Storage

After reconstitution, the Zebrafish Ndr1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.