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Home >> Antibodies >> Zebrafish Ncald Antibody / Isoforms a & b / Neurocalcin-delta A/B

Zebrafish Ncald Antibody / Isoforms a & b / Neurocalcin-delta A/B (RZ1005)

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  Catalog No Formulation Size Price (USD)  
Image RZ1005 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 539
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Western blot analysis of Ncalda/b protein using Ncald antibody and 1) zebrafish head 2) whole female zebrafish, 3) whole male zebrafish and 4) zebrafish embryo tissue lysate. Expected molecular weight ~22 kDa.
Western blot analysis of Ncalda/b protein using Ncald antibody and 1) zebrafish head 2) whole female zebrafish, 3) whole male zebrafish and 4) zebrafish embryo tissue lysate. Expected molecular weight ~22 kDa.
Availability 2-3 weeks
Species Reactivity Zebrafish
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity chromatography
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt A9JTH1 , Q6AXL4
Localization Cytoplasm, Nucleus
Applications Western Blot : 0.5-1 ug/ml
Limitations This Zebrafish Ncald antibody is available for research use only.
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Description

This gene encodes a member of the neuronal calcium sensor (NCS) family of calcium-binding proteins. The protein contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein is cytosolic at resting calcium levels; however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. The protein is thought to be a regulator of G protein-coupled receptor signal transduction. Several alternatively spliced variants of this gene have been determined, all of which encode the same protein; additional variants may exist but their biological validity has not been determined.

Application Notes

Optimal dilution of the Zebrafish Ncald antibody should be determined by the researcher.

Immunogen

An E.coli-derived zebrafish Ncalda/b recombinant protein (amino acids G2-F193) was used as the immunogen for the Zebrafish Ncald antibody. This antibody will detect the a and b isoforms.

Storage

After reconstitution, the Zebrafish Ncald antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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