Zebrafish MYL7 Antibody / Myosin Light Chain 7 Antibody (RZ1390)

Zebrafish MYL7 Antibody Muscle IHC. Immunohistochemical staining of paraffin-embedded zebrafish muscle tissue using Zebrafish MYL7 Antibody / Myosin Light Chain 7 Antibody demonstrates diffuse HRP-DAB brown staining throughout striated muscle fibers. The staining pattern is consistent with expression of MYL7, a myosin light chain family member involved in regulation of actomyosin interactions and muscle contractility. MYL7 is an important component of the contractile apparatus and is widely used as a marker of cardiac muscle differentiation and myocardial development in zebrafish. The observed fiber-associated staining is consistent with localization of myosin-associated proteins within organized contractile structures and supports the role of MYL7 in muscle function and sarcomere organization. Heat-mediated antigen retrieval was performed in EDTA buffer prior to overnight primary antibody incubation and HRP-DAB visualization.

Zebrafish MYL7 Antibody Cardiac Muscle IHC. Immunohistochemical staining of paraffin-embedded zebrafish heart tissue using Zebrafish MYL7 Antibody / Myosin Light Chain 7 Antibody demonstrates low-level cytoplasmic HRP-DAB brown staining within cardiac muscle tissue. MYL7 is a myosin light chain family member that functions within the cardiac contractile apparatus and contributes to regulation of actomyosin interactions, sarcomere organization, and myocardial contraction. In zebrafish, MYL7 is widely used as a marker of cardiomyocyte differentiation and heart development due to its association with developing and mature cardiac muscle cells. The observed staining pattern is consistent with expression of a contractile protein involved in cardiac muscle structure and function. Heat-mediated antigen retrieval was performed in EDTA buffer prior to overnight primary antibody incubation and HRP-DAB visualization.

Zebrafish MYL7 Antibody WB. Western blot analysis of zebrafish head tissue lysate using Zebrafish MYL7 Antibody / Myosin Light Chain 7 Antibody demonstrates a prominent band at approximately 17 kDa, consistent with the expected molecular weight of MYL7. Myosin Light Chain 7 (MYL7) is a contractile protein that forms part of the myosin motor complex and contributes to regulation of actomyosin interactions, sarcomere organization, and muscle contraction. In zebrafish, MYL7 is widely recognized as a marker of cardiac muscle differentiation and heart development, reflecting its association with cardiomyocyte function and myocardial maturation. The observed band confirms detection of MYL7 and supports the utility of this antibody for studies of cardiac development, muscle biology, and contractile protein expression. Additional faint higher molecular weight bands may represent myosin-associated protein complexes, post-translationally modified protein species, or other factors influencing electrophoretic migration. Electrophoresis was performed on a 12% SDS-PAGE gel under reducing conditions with 30 µg of protein loaded per lane, followed by nitrocellulose transfer and HRP-ECL detection.