Zebrafish Mcad Antibody / Acadm (RZ1138)

Description

Medium-chain acyl-CoA dehydrogenase (MCAD) is a mitochondrial enzyme that plays a central role in the β-oxidation of medium-chain fatty acids. Encoded by the acadm gene, MCAD catalyzes the initial dehydrogenation step in the metabolism of fatty acids with chain lengths of 6 to 12 carbons, producing enoyl-CoA derivatives and transferring electrons to the electron transport chain via electron transfer flavoprotein (ETF).

In zebrafish (Danio rerio), MCAD is highly conserved and functionally analogous to its mammalian counterparts, making it an ideal model for studying fatty acid metabolism and mitochondrial energy homeostasis in vertebrates. Zebrafish mcad is expressed during early development and in metabolically active tissues such as muscle, liver, and heart. Disruption of mcad function in zebrafish has been used to model human MCAD deficiency, a common inherited metabolic disorder that can lead to hypoglycemia, lethargy, and sudden infant death.

Due to its evolutionary conservation and physiological relevance, zebrafish MCAD is a valuable tool for research into metabolic diseases, mitochondrial function, and energy regulation in vivo.

Application Notes

Optimal dilution of the Zebrafish Mcad antibody should be determined by the researcher.

Immunogen

An E.coli-derived zebrafish Acadm/Mcad recombinant protein (amino acids S43-E406) was used as the immunogen for the Zebrafish Mcad antibody.

Storage

After reconstitution, the Zebrafish Mcad antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.