- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Zebrafish Lsg1 Antibody / Large subunit GTPase 1 (RZ1155)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RZ1155 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 539 |
| Availability | 2-3 weeks |
| Species Reactivity | Zebrafish |
| Format | Antigen affinity purified |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit Ig |
| Purity | Antigen affinity chromatography |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| UniProt | Q6NY89 |
| Localization | Cytoplasm |
| Applications | Western Blot : 0.5-1 ug/ml Immunohistochemistry (FFPE) : 2-5 ug/ml |
| Limitations | This Zebrafish Lsg1 antibody is available for research use only. |
|
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Zebrafish (Danio rerio) Lsg1 antibody detects Lsg1, a highly conserved GTPase required for the maturation and cytoplasmic processing of the 60S large ribosomal subunit. Encoded in zebrafish by the lsg1 gene, Large subunit GTPase 1 participates in late steps of ribosome biogenesis, ensuring that 60S subunits are correctly assembled, released from nuclear export factors, and rendered competent for translation. Because ribosome production is essential for protein synthesis, growth, and developmental progression, Zebrafish Lsg1 antibody reagents support research in translational control, ribosome assembly, and vertebrate cell growth.
Lsg1 functions as a quality-control checkpoint during cytoplasmic maturation of the 60S subunit. After nuclear export, immature ribosomal particles retain assembly factors that must be removed before functional ribosomes can join with 40S subunits to initiate translation. Lsg1 hydrolyzes GTP to release Nmd3, a required export adapter, thereby completing the maturation cycle and enabling subunit joining. In zebrafish embryos, lsg1 expression is present in rapidly proliferating and transcriptionally active tissues such as the brain, somites, endodermal organs, and developing musculature. These tissues demand high rates of ribosome production to support protein synthesis during growth and differentiation.
Defects in 60S subunit maturation can disrupt global translation and alter gene expression programs critical for development. Because the ribosome plays a central role in regulating protein output, Lsg1 contributes indirectly to pathways governing cell proliferation, lineage commitment, and metabolic adaptation. In zebrafish, impaired lsg1 function can reduce growth rates, increase cellular stress, or cause mispatterning in organ systems that require rapid protein synthesis. These phenotypes align with findings in other vertebrates, underscoring the conserved role of Large subunit GTPase 1 in ribosomal quality control.
Ribosome biogenesis is closely tied to developmental timing and nutrient-sensing pathways. In proliferating cells, growth regulators such as mTOR coordinate rRNA transcription, ribosomal protein synthesis, and assembly factor activity. Lsg1 acts downstream in the maturation phase, ensuring that newly produced large subunits are properly processed and capable of supporting high translational demand. Zebrafish embryos, which undergo rapid tissue expansion, rely on efficient Lsg1 activity to sustain structural protein production, metabolic enzyme synthesis, and the generation of developmental regulators.
At the molecular level, Lsg1 contains conserved GTP-binding motifs that enable nucleotide-dependent conformational changes necessary for release of assembly factors. Structural studies in other organisms show that Lsg1 engages the intersubunit face of the 60S particle, positioning itself to regulate late maturation steps. Subcellular localization in zebrafish is predominantly cytoplasmic, with enrichment near ribosome assembly intermediates. This localization pattern makes Lsg1 a useful marker for studying ribosomal maturation and cytoplasmic quality-control pathways.
Lsg1 also participates in maintaining ribosome homeostasis under stress. When translation is perturbed or ribosome production becomes imbalanced, Lsg1 and other GTPases modulate assembly activity to restore equilibrium. These mechanisms are particularly important during zebrafish development, where fluctuating metabolic conditions or environmental stressors may influence translational output.
A Zebrafish Lsg1 antibody is suitable for research applications such as western blotting, immunohistochemistry, and assays examining ribosome assembly, translational control, and cytoplasmic maturation of ribosomal subunits. This antibody targets Lsg1 for studies involving developmental growth, protein synthesis regulation, and vertebrate ribosome biology. NSJ Bioreagents provides the Zebrafish Lsg1 antibody to support research in translational regulation and cellular physiology.
Optimal dilution of the Zebrafish Lsg1 antibody should be determined by the researcher.
An E.coli-derived zebrafish Lsg1 recombinant protein (amino acids R21-G467) was used as the immunogen for the Zebrafish Lsg1 antibody.
After reconstitution, the Zebrafish Lsg1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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