- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Zebrafish Lmo2 Antibody / Rhombotin 2 (RZ1118)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RZ1118 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 539 |
|
| Availability | 2-3 weeks |
| Species Reactivity | Zebrafish |
| Format | Antigen affinity purified |
| Host | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit Ig |
| Purity | Antigen affinity chromatography |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| UniProt | Q9PTJ3 |
| Applications | Western Blot : 0.5-1 ug/ml |
| Limitations | This Zebrafish Lmo2 antibody is available for research use only. |
|
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Zebrafish (Danio rerio) Lmo2 antibody detects Lmo2, a LIM-domain containing transcriptional regulator essential for hematopoietic development, vascular formation, and early embryonic patterning. The zebrafish lmo2 gene encodes the ortholog of vertebrate Rhombotin 2, a protein best known for its role in assembling multi-protein transcriptional complexes that govern lineage specification in blood and endothelial progenitors. Lmo2 lacks a direct DNA-binding domain; instead, it exerts regulatory control by bridging DNA-binding transcription factors and co-regulators, enabling combinatorial gene expression programs central to early hematopoiesis. Because these pathways are deeply conserved across vertebrates, Zebrafish Lmo2 antibody reagents are widely used in studies examining blood development, vascular biology, and transcriptional regulation.
During zebrafish embryogenesis, lmo2 is expressed prominently in hematopoietic stem and progenitor cell territories, including the intermediate cell mass, posterior blood island, and later the kidney marrow. Its expression also extends to endothelial precursors that contribute to major vessels such as the dorsal aorta and cardinal vein. These overlapping roles reflect Lmo2's dual function in both hematopoietic and vascular specification. In vertebrate models, Lmo2 is required for the formation of hemangioblast-like precursors, establishing the developmental link between blood and vascular lineages. Zebrafish studies demonstrate that reducing lmo2 activity disrupts erythropoiesis, vasculogenesis, and angiogenic patterning.
At the molecular level, Lmo2 forms complexes with transcription factors including Tal1/Scl, Ldb1, Gata2, and E2A family proteins. These assemblies bind regulatory DNA elements that activate or repress lineage-specific transcriptional programs. Through interactions with these partners, Rhombotin 2 influences the balance between multipotency and commitment in hematopoietic progenitors. The Lmo2 complex regulates genes involved in erythroid differentiation, stem cell maintenance, and endothelial cell organization. In zebrafish, similar regulatory circuits operate during the emergence and expansion of early blood cells, contributing to the accessibility of the model for studying vertebrate hematopoiesis.
Lmo2 also has roles outside classical hematopoietic tissues. Expression in the developing nervous system and other embryonic tissues suggests broader contributions to cell differentiation and pattern formation. Although these functions are less characterized, they highlight the architectural flexibility of LIM-domain transcriptional scaffolds and their potential involvement in diverse developmental pathways. Zebrafish, with transparent embryos and tractable genetics, allow real-time visualization of Lmo2-driven processes during lineage specification, vascular remodeling, and organogenesis.
Subcellular localization of Lmo2 is primarily nuclear due to its role in transcriptional regulation. However, its correct localization depends on interactions with LIM-domain binding proteins and other transcriptional partners. The modular nature of the LIM domains enables recruitment of co-factors and structural integration into multi-protein assemblies that fine-tune regulatory outputs. Known interaction partners in zebrafish parallel those characterized in mammals, supporting the strong evolutionary conservation of Lmo2 function.
A Zebrafish Lmo2 antibody is suitable for research applications such as western blotting, immunohistochemistry, and assays examining hematopoietic development, vascular differentiation, and transcription factor complex assembly. This antibody targets Lmo2 for studies involving blood lineage formation, endothelial patterning, and vertebrate developmental regulation. NSJ Bioreagents provides the Zebrafish Lmo2 antibody to support research in hematopoiesis, vascular biology, and transcriptional control mechanisms.
Optimal dilution of the Zebrafish Lmo2 antibody should be determined by the researcher.
An E.coli-derived zebrafish Lmo2 recombinant protein (amino acids E14-A124) was used as the immunogen for the Zebrafish Lmo2 antibody.
After reconstitution, the Zebrafish Lmo2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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