Zebrafish IRF6 Antibody / Interferon Regulatory Factor 6 (RZ1459)

Zebrafish IRF6 Antibody Brain IHC. Immunohistochemical analysis of IRF6 expression was performed using anti-IRF6 antibody in paraffin-embedded zebrafish brain tissue. IRF6, also known as Interferon Regulatory Factor 6, is a transcription factor involved in epithelial differentiation, tissue morphogenesis, and developmental signaling. Moderate cytoplasmic and nuclear staining is observed in neural cells, consistent with the role of IRF6 in regulating gene expression and maintaining tissue homeostasis during vertebrate development. The widespread staining pattern within brain tissue suggests that IRF6 contributes to cellular differentiation and developmental processes beyond its established roles in craniofacial morphogenesis. These results support the utility of Zebrafish IRF6 Antibody for studies of developmental biology, epithelial differentiation, and tissue regeneration. Heat-mediated antigen retrieval was performed in EDTA buffer, and sections were blocked with 10% goat serum before incubation with IRF6 antibody at 2 ug/ml overnight at 4°C. Detection was achieved using peroxidase-conjugated goat anti-rabbit IgG and DAB chromogen.

Zebrafish IRF6 Antibody WB. Western blot analysis of IRF6 expression was performed using anti-IRF6 antibody. Electrophoresis was carried out on a 10% SDS-PAGE gel under reducing conditions. Lane 1: zebrafish head tissue lysate. Lane 2: zebrafish embryo tissue lysate. IRF6, also known as Interferon Regulatory Factor 6, is a developmental transcription factor that regulates epithelial differentiation, tissue morphogenesis, and craniofacial development. A specific immunoreactive band is detected at approximately 60 kDa in both samples, close to the expected molecular weight of IRF6 (~55 kDa). Expression in head and embryo lysates is consistent with the important role of IRF6 during embryogenesis and tissue patterning. These results support the utility of Zebrafish IRF6 Antibody for studies of developmental biology, epithelial differentiation, and vertebrate morphogenesis.