- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Zebrafish ILF2 Antibody / Interleukin enhancer-binding factor 2 / NF45 (RZ1039)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RZ1039 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 539 |
| Availability | 2-3 weeks |
| Species Reactivity | Zebrafish |
| Format | Antigen affinity purified |
| Host | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit Ig |
| Purity | Antigen affinity chromatography |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| UniProt | Q6NZ06 |
| Localization | Cytoplasm, Nucleus |
| Applications | Western Blot : 0.5-1 ug/ml Immunohistochemistry (FFPE) : 2-5 ug/ml Immunofluorescence : 5ug/ml |
| Limitations | This Zebrafish ILF2 antibody is available for research use only. |
|
Review this product on BioCompare and get a $20 Amazon gift card
|
Related Products
|
Zebrafish (Danio rerio) ILF2 antibody recognizes Interleukin enhancer-binding factor 2, also known as NF45, a conserved RNA binding and transcription-regulatory protein encoded by the zebrafish ilf2 gene. ILF2 forms a stable heterodimer with ILF3 (NF90), together functioning in multiple aspects of RNA metabolism, transcriptional regulation, DNA repair, and immune-related gene expression. In Danio rerio, ILF2 is widely expressed during embryogenesis with enrichment in proliferative tissues including the developing brain, neural tube, somites, craniofacial mesenchyme, hematopoietic regions, and early endodermal organs. Subcellular localization is predominantly nuclear but includes cytoplasmic pools that participate in RNA processing and transport.
Interleukin enhancer-binding factor 2 plays a central role in coordinating transcription and RNA handling during rapid developmental transitions. ILF2 contributes to transcriptional activation at promoters regulated by cytokine-responsive or developmental transcription factors. In zebrafish, ILF2 is required for the correct expression of genes that shape neural patterning, hematopoietic lineage commitment, and mesodermal organization. Because early zebrafish tissues undergo rapid cell division and extensive transcriptional remodeling, ILF2-mediated stabilization of RNA-protein complexes supports gene expression fidelity.
ILF2 also participates in mRNA stabilization, nuclear export, and the assembly of ribonucleoprotein particles. Together with ILF3, ILF2 binds structured regions of target mRNAs to regulate transcript half-life and translational competence. During zebrafish development, these post transcriptional mechanisms influence cell cycle progression, neural crest differentiation, muscle precursor development, and early immune system formation. ILF2-dependent RNA regulation is especially important in neural progenitor populations where precise expression of differentiation regulators is required for proper brain and spinal cord patterning.
ILF2 contributes to DNA damage responses and genome maintenance. In vertebrate systems, ILF2 participates in double-strand break repair, checkpoint signaling, and chromatin-associated stress responses. These functions appear conserved in zebrafish, where ILF2 activity supports resilience during periods of high replication stress in early embryos. Developing tissues that rely on robust proliferation, such as retina, brain ventricles, and hematopoietic sites, require efficient ILF2-mediated regulatory mechanisms to maintain genomic stability.
ILF2 also influences immune-related transcriptional programs. Although zebrafish embryos lack a fully mature adaptive immune system, ILF2 participates in early innate immune signaling and cytokine-responsive gene regulation. NF45 has been implicated in controlling the expression of factors involved in inflammatory signaling, antiviral defense, and stress adaptation, processes that are increasingly studied using zebrafish as a transparent, genetically tractable vertebrate model. ILF2 helps modulate gene networks linked to NF-kappaB activity, interferon responses, and stress-induced transcriptional reprogramming.
At the molecular level, ILF2 contains RNA binding domains, coactivator motifs, and regulatory regions that interact with ILF3, transcription factors, and chromatin-associated proteins. Isoform diversity or differential regulation may fine-tune ILF2 activity across embryonic tissues. Its dual involvement in transcriptional activation and RNA regulation positions ILF2 as a key integrator of nuclear processes that support rapid developmental progression.
This Zebrafish ILF2 antibody is suitable for detecting NF45 in research focused on transcriptional control, RNA processing, neural development, hematopoiesis, DNA repair, and innate immune signaling in zebrafish. It supports studies examining ILF2-ILF3 ribonucleoprotein complexes, promoter activation mechanisms, and developmental phenotypes resulting from altered RNA-protein regulation. NSJ Bioreagents provides this reagent within its zebrafish and RNA regulation antibody collection.
Optimal dilution of the Zebrafish ILF2 antibody should be determined by the researcher.
An E.coli-derived zebrafish NF45/ILF2 recombinant protein (amino acids Y23-K356) was used as the immunogen for the Zebrafish ILF2 antibody.
After reconstitution, the Zebrafish ILF2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Your bulk quote request has been submitted successfully!
Please contact us if you have any questions.
Powered by Bioz
