Zebrafish IL-4 Antibody (RZ1348)

Zebrafish IL-4 Antibody IHC. Immunohistochemistry analysis of IL4 using anti-IL4 antibody. IL4 was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-IL4 Antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using DAB as the chromogen.

Zebrafish IL-4 Antibody IHC. Immunohistochemistry analysis of IL4 using anti-IL4 antibody. IL4 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-IL4 Antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using DAB as the chromogen.

Zebrafish IL-4 Antibody IHC. Immunohistochemistry analysis of IL4 using anti-IL4 antibody. IL4 was detected in a paraffin-embedded section of zebrafish bone tissue. Heat mediated antigen retrieval was performed in EDTA buffer. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-IL4 Antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using DAB as the chromogen.

Zebrafish IL-4 Antibody WB. Western blot analysis of IL4 using anti-IL4 antibody. Electrophoresis was performed on a 12% SDS-PAGE gel for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL4 antigen antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus WB. Western blotting Substrate with Tanon 5200 system. A specific band was detected for IL4 at approximately 14 kDa. The expected band size for IL4 is at 14 kDa.