Zebrafish IkB Alpha Antibody / NFKBIAA Antibody (RZ1351)

Zebrafish IkB Alpha / NFKBIAA Antibody Kidney IHC. Immunohistochemistry staining of FFPE zebrafish kidney tissue using Zebrafish IkB Alpha Antibody demonstrates cytoplasmic HRP-DAB brown staining within renal tubular epithelial cells and associated cellular populations. The staining pattern is consistent with expression of Nuclear Factor of Kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor Alpha A (NFKBIAA), also known as IkB Alpha, a key negative regulator of NF-kB signaling that controls inflammatory, immune, and stress-response pathways. Prominent staining within renal epithelial structures is consistent with the established role of NF-kB regulatory proteins in maintaining tissue homeostasis and coordinating cellular responses to physiological and environmental stimuli. Heat mediated antigen retrieval was performed in EDTA buffer. Primary antibody was incubated overnight at 4°C followed by detection using a peroxidase-conjugated goat anti-rabbit secondary antibody and DAB chromogen.

Zebrafish IkB Alpha / NFKBIAA Antibody NF-kB Signaling WB. Western blot analysis of zebrafish head tissue and whole female zebrafish lysates using Zebrafish IkB Alpha Antibody demonstrates a prominent immunoreactive band at approximately 35 kDa, consistent with the predicted molecular weight of Nuclear Factor of Kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor Alpha A (NFKBIAA), also known as IkB Alpha. Additional lower and higher molecular weight immunoreactive bands are observed and may represent modified forms, degradation products, or other antibody-reactive species. NFKBIAA is a key negative regulator of NF-kB signaling that controls inflammatory, immune, and cellular stress-response pathways through sequestration of NF-kB transcription factors in the cytoplasm. Electrophoresis was performed on a 10% SDS-PAGE gel under reducing conditions followed by transfer to a nitrocellulose membrane. Signal was detected using an HRP-conjugated secondary antibody and enhanced chemiluminescent substrate.