Zebrafish IFNPHI3 Antibody (RZ1343)

Zebrafish IFNPHI3 Antibody IHC. Immunohistochemistry analysis of IFNPHI3 using anti-IFNPHI3 antibody. IFNPHI3 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-IFNPHI3 Antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using DAB as the chromogen.

Zebrafish IFNPHI3 Antibody WB. Western blot analysis of IFNPHI3 using anti-IFNPHI3 antibody. Electrophoresis was performed on a 12% SDS-PAGE gel for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFNPHI3 antigen antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus WB. Western blotting Substrate with Tanon 5200 system. A specific band was detected for IFNPHI3 at approximately 21 kDa. The expected band size for IFNPHI3 is at 21 kDa.