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Home >> Antibodies >> Zebrafish Hsp60 Antibody / Hspd1

Zebrafish Hsp60 Antibody / Hspd1 (RZ1163)

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Image RZ1163 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 539
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Western blot testing of zebrafish embryo tissue lysate with Zebrafish Hsp60 antibody. Predicted molecular weight ~61 kDa.
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Western blot testing of zebrafish embryo tissue lysate with Zebrafish Hsp60 antibody. Predicted molecular weight ~61 kDa.
Immunofluorescent analysis of Hsp60/Hspd1 protein using Zebrafish Hsp60 antibody (green) and DAPI nuclear stain (blue) with zebrafish embryo tissue.
IHC staining of FFPE zebrafish heart tissue with Zebrafish Hsp60 antibody, HRP secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE zebrafish liver tissue with Zebrafish Hsp60 antibody, HRP secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE zebrafish spinal cord tissue with Zebrafish Hsp60 antibody, HRP secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE zebrafish kidney tissue with Zebrafish Hsp60 antibody, HRP secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Availability 2-3 weeks
Species Reactivity Zebrafish
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity chromatography
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q803B0
Localization Cytoplasm
Applications Western Blot : 0.5-1 ug/ml
Immunohistochemistry (FFPE) : 2-5 ug/ml
Immunofluorescence : 5 ug/ml
Limitations This Zebrafish Hsp60 antibody is available for research use only.
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Description

HSP60 is a member of the chaperonin class of protein factors, which include the Escherichia coli groEL protein and the Rubisco subunit-binding protein of chloroplasts. It acts as a costimulator of human regulatory CD4-positive/CD25 -positive T cells, which inhibit lymphoproliferation and IFNG and TNF secretion by CD4-positive and CD8-positive T cells. HSP60 enhances Treg activity via TLR2, leading to activation of an intracellular signaling cascade that included p38, as well as inhibition of ERK phosphorylation. Suppression of target T cells is mediated by both cell-to-cell contact and by secretion of TGFB and IL10, and it leads to downregulation of ERK, NFKB, and TBET expression. The self-molecule HSP60 can downregulate adaptive immune responses by upregulating Tregs through TLR2 signaling.

Application Notes

Optimal dilution of the Zebrafish Hsp60 antibody should be determined by the researcher.

Immunogen

An E.coli-derived zebrafish HSP60/HSPD1 recombinant protein (amino acids K72-Q496) was used as the immunogen for the Zebrafish Hsp60 antibody.

Storage

After reconstitution, the Zebrafish Hsp60 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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