- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Zebrafish Hsp60 Antibody / Hspd1 (RZ1163)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RZ1163 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 539 |
| Availability | 2-3 weeks |
| Species Reactivity | Zebrafish |
| Format | Antigen affinity purified |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit Ig |
| Purity | Antigen affinity chromatography |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| UniProt | Q803B0 |
| Localization | Cytoplasm |
| Applications | Western Blot : 0.5-1 ug/ml Immunohistochemistry (FFPE) : 2-5 ug/ml Immunofluorescence : 5 ug/ml |
| Limitations | This Zebrafish Hsp60 antibody is available for research use only. |
|
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Zebrafish (Danio rerio) Hsp60 antibody detects Hsp60, a highly conserved mitochondrial chaperonin essential for proper protein folding, mitochondrial biogenesis, and cellular stress resilience. Encoded in zebrafish by the hspd1 gene, Hsp60 functions within the mitochondrial matrix where it assists in the folding, refolding, or stabilization of proteins imported from the cytosol. As a key member of the heat shock protein family, Hsp60 supports proteostasis under both basal and stress-induced conditions. Because mitochondrial performance is central to vertebrate development, metabolism, and survival, Zebrafish Hsp60 antibody reagents support research in protein quality control, mitochondrial physiology, stress signaling, and developmental bioenergetics.
Hsp60 forms a barrel-shaped oligomeric complex that provides an isolated environment for protein folding. Working in conjunction with its co-chaperonin Hsp10, Hsp60 facilitates ATP-dependent conformational cycling that allows unfolded or misfolded substrates to achieve native structure. In zebrafish embryos, hspd1 expression is enriched in tissues with high mitochondrial content or metabolic rates, including skeletal muscle, heart, brain, and rapidly proliferating organ primordia. These tissues rely on efficient chaperonin activity to maintain mitochondrial proteome integrity during periods of growth and differentiation.
During development, mitochondrial proteins face increased folding demands due to rapid organogenesis, elevated biosynthetic activity, and dynamic metabolic shifts. Hsp60 assists in the assembly and stabilization of metabolic enzymes, respiratory chain components, and mitochondrial ribosomal proteins. Disruption of hspd1 impairs mitochondrial function, reduces ATP output, and can lead to widespread developmental abnormalities. Zebrafish offer a powerful model for examining how Hsp60-mediated quality control contributes to organ development, metabolic adaptation, and tissue-specific mitochondrial biology.
Hspd1 is also central to cellular stress responses. Under heat shock, oxidative stress, or metabolic perturbation, misfolded proteins accumulate within mitochondria, triggering compensatory upregulation of Hsp60. This response protects cells from proteotoxicity by restoring proteostasis and preserving mitochondrial membrane potential. In zebrafish, Hsp60 upregulation occurs during environmental stress, developmental transitions, and disease-model conditions, making it a valuable marker for mitochondrial stress and adaptive proteostasis pathways.
Beyond its mitochondrial roles, Hsp60 has been implicated in cytosolic and extracellular signaling in several vertebrate systems. While primarily localized to mitochondria, Hsp60 can influence apoptosis, immune activation, and cellular communication depending on context. Although these functions are less explored in zebrafish, the conservation of Hspd1 suggests similar regulatory potential.
At the molecular level, Hsp60 consists of equatorial, intermediate, and apical domains that mediate ATP binding, oligomer assembly, and substrate encapsulation. The chaperonin undergoes ATP-driven conformational changes that regulate substrate engagement and release. These dynamic features allow Hsp60 to accommodate a broad range of folding substrates required for mitochondrial maintenance and recovery from stress.
A Zebrafish Hsp60 antibody is suitable for research applications such as western blotting, immunohistochemistry, and assays examining mitochondrial protein folding, stress responses, and organ development. This antibody targets Hsp60 for studies involving proteostasis, metabolic regulation, and mitochondrial quality-control mechanisms. NSJ Bioreagents provides the Zebrafish Hsp60 antibody to support research in mitochondrial biology and developmental physiology.
Optimal dilution of the Zebrafish Hsp60 antibody should be determined by the researcher.
An E.coli-derived zebrafish HSP60/HSPD1 recombinant protein (amino acids K72-Q496) was used as the immunogen for the Zebrafish Hsp60 antibody.
After reconstitution, the Zebrafish Hsp60 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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