- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Zebrafish Hsd3b1 Antibody / 3-beta-hydroxysteroid dehydrogenase type 1 (RZ1198)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RZ1198 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 539 |
| Availability | 2-3 weeks |
| Species Reactivity | Zebrafish |
| Format | Antigen affinity purified |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit Ig |
| Purity | Antigen affinity chromatography |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| UniProt | F1QSA2 |
| Localization | Cytoplasm |
| Applications | Western Blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml |
| Limitations | This Zebrafish Hsd3b1 antibody is available for research use only. |
|
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Zebrafish Hsd3b1 antibody detects Hsd3b1, an essential enzyme in the steroidogenic pathway responsible for converting delta-5 steroids into delta-4 ketosteroids. This reaction represents a critical early step in the biosynthesis of progesterone, androgens, estrogens, and glucocorticoids. In zebrafish (Danio rerio), Hsd3b1 functions within steroid-producing tissues to regulate hormonal output required for development, reproduction, and stress physiology. Known in the literature as 3-beta-hydroxysteroid dehydrogenase type 1, Hsd3b1 plays conserved roles across vertebrates in coordinating endocrine signaling and maintaining steroid hormone balance. Because steroidogenesis governs processes from gonadal differentiation to stress adaptation, Zebrafish Hsd3b1 antibody reagents support research in endocrinology, developmental biology, and metabolic regulation.
During zebrafish development, hsd3b1 is expressed in interrenal tissue, gonads, brain regions associated with neuroendocrine control, and specific embryonic domains involved in steroid production. In developing embryos, Hsd3b1 activity influences cortisol synthesis, which in turn regulates stress-axis maturation and metabolic readiness. The interrenal gland in zebrafish, analogous to the mammalian adrenal cortex, depends on Hsd3b1 expression for proper steroidogenic function. In scientific literature, zebrafish proteins such as Hsd3b1 are frequently referenced using Danio rerio naming, and terms like Danio Hsd3b1 or Danio rerio Hsd3b1 appear interchangeably with zebrafish terminology.
3-beta-hydroxysteroid dehydrogenase type 1 is also a key driver of gonadal steroid production. In testes, Hsd3b1 supports androgen biosynthesis required for spermatogenesis and male sexual development. In ovaries, Hsd3b1 contributes to progesterone and estrogen pathways that regulate oocyte maturation, follicular development, and reproductive cycling. Disruption of hsd3b1 can impair steroid balance, alter reproductive outcomes, and affect sex differentiation in zebrafish, making it a critical enzyme for studying endocrine regulation.
Beyond reproductive roles, Hsd3b1 is involved in neural and behavioral processes influenced by steroid hormones. It contributes to neuroendocrine signaling and may influence stress-axis sensitivity, behavioral responses, and metabolic adjustments. In peripheral tissues, including liver and gill epithelium, Hsd3b1 participates in metabolic steroid processing relevant to environmental adaptation.
At the molecular and biochemical levels, Hsd3b1 catalyzes two sequential reactions: the oxidation of a 3-beta-hydroxyl group and the isomerization of a delta-5 double bond. These reactions occur in the endoplasmic reticulum and rely on cofactor interactions to support efficient steroid conversion. Subcellular localization and activity are regulated by developmental cues, nutritional state, environmental conditions, and endocrine feedback loops.
Zebrafish models offer valuable advantages for studying Hsd3b1 function, including transparent embryos, accessible tissue architecture, and sensitivity to steroidogenic disruption. Because endocrine-disrupting chemicals can affect Hsd3b1 expression or activity, zebrafish serve as an informative system for toxicology and environmental health research. Hsd3b1 also provides insights into the evolution of steroidogenic enzymes across vertebrates.
A Zebrafish Hsd3b1 antibody is suitable for research applications such as western blotting, immunohistochemistry, and assays examining steroidogenesis, endocrine signaling, reproductive development, and stress physiology. This antibody targets 3-beta-hydroxysteroid dehydrogenase type 1 for studies involving metabolic and hormonal regulation. NSJ Bioreagents provides the Zebrafish Hsd3b1 antibody to support research in endocrine and developmental biology.
Optimal dilution of the Zebrafish Hsd3b1 antibody should be determined by the researcher.
E. coli-derived zebrafish Hsd3b1 recombinant protein (amino acids F16-K374) was used as the immunogen for the Zebrafish Hsd3b1 antibody.
After reconstitution, the Zebrafish Hsd3b1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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