Zebrafish HOXA3 Antibody / Homeobox Protein Hox-A3a Antibody (RZ1466)

Zebrafish HOXA3 Antibody Liver IHC. Immunohistochemical analysis of HOXA3 expression was performed using anti-HOXA3A antibody in paraffin-embedded zebrafish liver tissue. HOXA3A is a zebrafish ortholog of the mammalian HOXA3 homeobox transcription factor that regulates embryonic patterning, tissue specification, and developmental gene expression. Moderate nuclear and cytoplasmic staining is observed in hepatocytes, consistent with the role of HOXA3A in controlling developmental transcriptional programs and maintaining tissue-specific gene expression. Although HOXA3 is best known for its functions during embryogenesis, continued expression in adult tissues supports roles in cellular differentiation and tissue homeostasis. These results demonstrate the utility of Zebrafish HOXA3 Antibody for studies of developmental biology, transcriptional regulation, vertebrate morphogenesis, and HOX gene function. Heat-mediated antigen retrieval was performed in EDTA buffer, followed by incubation with HOXA3A antibody at 2 ug/ml overnight at 4°C. Detection was achieved using a peroxidase-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen.

Zebrafish HOXA3 Antibody Brain IHC. Immunohistochemical analysis of HOXA3 expression was performed using anti-HOXA3A antibody in paraffin-embedded zebrafish brain tissue. HOXA3A is a zebrafish ortholog of the mammalian HOXA3 homeobox transcription factor that regulates embryonic patterning, tissue specification, and developmental gene expression. Nuclear staining is observed in scattered neural cells, consistent with the function of HOXA3A as a DNA-binding transcription factor that controls developmental transcriptional programs. Expression within the nervous system supports the established role of HOX proteins in vertebrate neural development, regional identity, and maintenance of differentiated cell populations. These results demonstrate the utility of Zebrafish HOXA3 Antibody for studies of developmental biology, neurodevelopment, transcriptional regulation, and HOX gene function. Heat-mediated antigen retrieval was performed in EDTA buffer, followed by incubation with HOXA3A antibody at 2 ug/ml overnight at 4°C. Detection was achieved using a peroxidase-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen.

Zebrafish HOXA3 Antibody WB. Western blot analysis of HOXA3 expression was performed using anti-HOXA3A antibody. Electrophoresis was carried out on a 10% SDS-PAGE gel under reducing conditions. Lane 1: zebrafish head tissue lysate. Lane 2: whole female zebrafish tissue lysate. Lane 3: whole male zebrafish tissue lysate. HOXA3A is a zebrafish ortholog of the mammalian HOXA3 homeobox transcription factor that regulates embryonic patterning, tissue specification, and developmental gene expression. A specific immunoreactive band is detected at approximately 50 kDa in all three samples, slightly above the predicted molecular weight of approximately 44 kDa, while lower molecular weight bands are also observed and may represent processed forms or non-specific reactivity. The consistent detection of the predominant HOXA3A band across head and whole-body lysates supports the widespread developmental role of this transcription factor in regulating vertebrate morphogenesis and cellular differentiation. These results demonstrate the utility of Zebrafish HOXA3 Antibody for studies of developmental biology, transcriptional regulation, embryonic patterning, and HOX gene function.