- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Zebrafish Hook3 Antibody (RZ1135)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RZ1135 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 539 |
| Availability | 2-3 weeks |
| Species Reactivity | Zebrafish |
| Format | Antigen affinity purified |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit Ig |
| Purity | Antigen affinity chromatography |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| UniProt | A0A8M6Z0P5 |
| Applications | Immunohistochemistry (FFPE) : 2-5 ug/ml |
| Limitations | This Zebrafish Hook3 antibody is available for research use only. |
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Zebrafish (Danio rerio) Hook3 antibody detects Hook3, a conserved microtubule-binding and vesicle-tethering protein involved in endosomal transport, organelle positioning, and intracellular trafficking. In zebrafish, the hook3 gene encodes a coiled-coil adaptor protein that links cargo-containing vesicles to the cytoskeleton, playing a key role in dynein-mediated minus-end transport. Hook proteins contribute to the organization of early endosomes, the movement of cargo toward the microtubule-organizing center, and the assembly of protein complexes that regulate endolysosomal maturation. Because these transport systems are critical for developmental signaling and cellular homeostasis, Zebrafish Hook3 antibody reagents support research in vesicular trafficking, neurodevelopment, and organelle dynamics.
Hook3 functions as part of the FHF complex (Fused Toes, Hook proteins, and FTS) and acts as a scaffold between endosomal membranes and the dynein-dynactin motor machinery. Through its N-terminal microtubule-binding domain and C-terminal cargo-binding region, Hook3 positions early endosomes along microtubules and coordinates their movement toward the perinuclear region. In zebrafish embryos, hook3 is expressed in neural lineages, epithelial tissues, somites, and developing organs that depend heavily on endosomal transport for signaling and morphogenesis. Proper Hook3 activity ensures that receptors and signaling molecules are trafficked efficiently, supporting precise developmental patterning.
Endosomal trafficking mediated by Hook3 influences multiple signaling pathways, including those regulated by Wnt, Notch, and receptor tyrosine kinases. These pathways rely on endosome maturation, receptor recycling, or degradation, and Hook3 plays a central role in organizing the endosomal compartments that determine signal duration and intensity. In zebrafish, impairment of hook3 function can lead to defects in neuronal differentiation, alterations in epithelial polarity, or disruptions in organ formation due to misregulated receptor trafficking.
In addition to its transport functions, Hook3 contributes to Golgi positioning and maintains perinuclear organelle architecture. Studies in vertebrate models show that Hook3 interacts with Golgi-associated proteins and participates in centrosome organization. These functions help establish intracellular polarity, especially in migrating cells or developing neurons. Because zebrafish embryos undergo extensive cell movement and rapid morphogenetic rearrangements, Hook3-dependent coordination of organelle positioning is particularly important during early development.
At the molecular level, Hook3 forms elongated dimers capable of binding both microtubules and vesicular membranes, creating a physical bridge that facilitates cargo movement. It interacts with dynein light intermediate chains, dynactin components, and endosomal markers such as Rab5. These partnerships enable Hook3 to regulate the rate and directionality of vesicle transport. Subcellular localization of Hook3 is enriched at early endosomes, the centrosome, and microtubule tracks, consistent with its roles in vesicle tethering and organelle organization.
Zebrafish provide a strong platform for visualizing Hook3 activity in vivo, especially within developing neural circuits and epithelial layers. Live-imaging studies highlight the dependence of axonal transport, synaptic vesicle trafficking, and organelle positioning on Hook family proteins. The conservation of Hook3 structure and function across vertebrates makes zebrafish an effective model for studying diseases associated with endosomal dysfunction, including neurodevelopmental and degenerative disorders.
A Zebrafish Hook3 antibody is suitable for research applications such as western blotting, immunohistochemistry, and assays investigating vesicle transport, endosomal organization, and intracellular trafficking. This antibody targets Hook3 for studies of cytoskeletal dynamics, motor protein regulation, and vertebrate developmental pathways. NSJ Bioreagents provides the Zebrafish Hook3 antibody to support research in membrane trafficking and cellular organization.
Optimal dilution of the Zebrafish Hook3 antibody should be determined by the researcher.
An E.coli-derived zebrafish Hook3 recombinant protein (amino acids E14-R198) was used as the immunogen for the Zebrafish Hook3 antibody.
After reconstitution, the Zebrafish Hook3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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