- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Zebrafish Hmgb1 Antibody / Hmgb1a / Hmgb1b (RZ1154)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RZ1154 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 539 |
| Availability | 2-3 weeks |
| Species Reactivity | Zebrafish |
| Format | Antigen affinity purified |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit Ig |
| Purity | Antigen affinity chromatography |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| UniProt | Q6NX86 , X1WFI9 |
| Localization | Nuclear, cytoplasmic, cell membrane, extracellular |
| Applications | Immunohistochemistry (FFPE) : 2-5 ug/ml Western Blot : 0.5-1ug/ml |
| Limitations | This Zebrafish Hmgb1 antibody is available for research use only. |
|
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Zebrafish (Danio rerio) Hmgb1 antibody detects Hmgb1, a highly conserved chromatin-binding protein that influences DNA architecture, transcriptional regulation, and innate immune signaling. In zebrafish, this activity is carried out by two paralogs, hmgb1a and hmgb1b, which encode proteins structurally and functionally similar to mammalian HMGB1. Hmgb1 belongs to the high mobility group box family, defined by two HMG-box DNA-binding domains that bend and stabilize DNA to facilitate transcription factor access, nucleosome remodeling, and DNA repair. Because Hmgb1 integrates roles in nuclear regulation and extracellular signaling, Zebrafish Hmgb1 antibody reagents support research in chromatin biology, inflammation, development, and tissue remodeling.
Inside the nucleus, Hmgb1 regulates transcription by modulating chromatin accessibility. It interacts with diverse transcription factors and DNA structures, enhancing the recruitment of regulatory proteins to promoter and enhancer regions. In zebrafish embryos, Hmgb1a and Hmgb1b are widely expressed in proliferative and differentiating tissues including the brain, somites, vasculature, and organs undergoing rapid morphogenesis. These expression domains reflect the need for dynamic chromatin regulation during early developmental patterning.
Hmgb1 also plays critical roles in DNA repair. By binding to damaged DNA and interacting with repair complexes, it assists pathways such as base excision repair and nucleotide excision repair. During zebrafish development, when cells undergo rapid division and experience replicative stress, proper Hmgb1 function helps maintain genomic stability and prevent accumulation of DNA lesions.
In addition to its nuclear functions, Hmgb1 can act extracellularly as a signaling molecule. When released from stressed or damaged cells, Hmgb1 functions as an alarmin that activates innate immune receptors including RAGE and TLRs, promoting inflammation, tissue repair, and regeneration. Zebrafish are widely used to study tissue injury responses, and Hmgb1 participates in wound healing, immune cell recruitment, and regenerative processes. Differences between Hmgb1a and Hmgb1b expression may contribute to paralog-specific functions in immune signaling or stress adaptation.
At the molecular level, Hmgb1 binds DNA in a sequence-independent manner, recognizing structural features such as bends, loops, or unwound regions. Its two HMG-box domains enable DNA bending and chromatin decompaction, supporting transcriptional activation and nucleosome mobility. Subcellular localization is primarily nuclear under homeostatic conditions, but Hmgb1 translocates to the cytoplasm or extracellular space during inflammatory or stress responses. This dynamic localization underscores its dual role as both a chromatin regulator and extracellular signaling factor.
Because Hmgb1 regulates transcriptional networks, participates in DNA repair, and modulates innate immunity, disruptions in its function can have broad effects on development. In zebrafish, altered hmgb1a or hmgb1b expression influences neurodevelopment, vascular formation, inflammation, and regenerative capacity. These conserved functions make zebrafish an excellent model for exploring Hmgb1 roles in development and disease.
A Zebrafish Hmgb1 antibody is suitable for research applications such as western blotting, immunohistochemistry, and assays examining chromatin dynamics, transcriptional regulation, immune signaling, and tissue repair. This antibody targets Hmgb1a and Hmgb1b for studies involving nuclear architecture, inflammation, and vertebrate developmental biology. NSJ Bioreagents provides the Zebrafish Hmgb1 antibody to support research in chromatin regulation and innate immunity.
Beyond its nuclear role, Hmgb1 can act as an extracellular signaling molecule involved in inflammation, immune responses, and tissue repair. The human ortholog of zebrafish Hmgb1 is HMGB1, which is highly conserved and serves similar functions in chromatin dynamics and extracellular signaling. This evolutionary conservation highlights its importance in both normal physiology and disease research.
Using a high-quality Zebrafish Hmgb1 antibody enables accurate detection in applications such as western blot, immunohistochemistry, and immunofluorescence. A Zebrafish Hmgb1 antibody from NSJ Bioreagents ensures sensitivity and reproducibility for studies of chromatin remodeling, immune signaling, and developmental biology. Selecting the right Zebrafish Hmgb1 antibody is essential for generating reliable and consistent results.
Optimal dilution of the Zebrafish Hmgb1 antibody should be determined by the researcher.
An E.coli-derived zebrafish Hmgb1a/b recombinant protein (amino acids D123-K153) was used as the immunogen for the Zebrafish Hmgb1 antibody. This antibody will detect the a and b isoforms.
After reconstitution, the Zebrafish Hmgb1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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