- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Zebrafish Histone H3 Antibody (RZ1070)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RZ1070 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 539 |
| Availability | 2-3 weeks |
| Species Reactivity | Zebrafish |
| Format | Antigen affinity purified |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit Ig |
| Purity | Antigen affinity chromatography |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| UniProt | Q6PI20 |
| Applications | Western Blot : 0.5-1 ug/ml Immunohistochemistry (FFPE) : 2-5 ug/ml Immunofluorescence : 5 ug/ml |
| Limitations | This Zebrafish Histone H3 antibody is available for research use only. |
|
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Zebrafish Histone H3 antibody recognizes the core nucleosomal protein Histone H3, a fundamental chromatin component encoded by several histone H3 gene variants in zebrafish. Histone H3 is essential for nucleosome assembly, chromatin compaction, and epigenetic regulation during transcription, DNA replication, and DNA repair. In Danio rerio embryos, histone H3 transcripts are abundant from early cleavage stages due to the rapid synthesis of chromatin required for successive cell cycles. With the onset of gastrulation and organogenesis, Histone H3 is widely expressed in developing brain, neural tube, somites, heart, vasculature, notochord, and endoderm-derived tissues including liver and pancreas. Subcellular localization is strictly nuclear, reflecting its central structural role in chromatin.
Histone H3 supports genome organization during rapid cell division. Zebrafish embryos undergo extremely fast cell cycles before the mid-blastula transition, requiring robust nucleosome assembly to package newly replicated DNA. As differentiation progresses, histone H3 variants and post translational modifications contribute to the establishment of stable or dynamic chromatin states that guide tissue-specific gene expression. Methylation, acetylation, and phosphorylation of histone H3 are key epigenetic markers associated with developmental lineage commitment, cell identity, and transcriptional regulation.
Neural development is highly dependent on histone H3-mediated chromatin remodeling. Neural progenitors require precise control of gene expression programs that define neuroepithelial organization, proliferation, and differentiation. Histone H3 modifications regulate accessibility of transcriptional regulators and influence patterns of neuronal maturation. During later stages, chromatin remodeling continues to drive synaptic development, axon guidance, and neural circuit formation. Perturbation of histone H3 dynamics can alter neurogenesis or disrupt regional brain patterning.
Somite and skeletal muscle development also rely on histone H3-dependent epigenetic mechanisms. Myogenic differentiation requires regulated expression of transcription factors and structural genes controlled by chromatin accessibility. Histone H3 modifications help establish transcriptional states needed for segmentation, myotome formation, and early muscle fiber organization. Because muscle progenitors transition quickly from proliferative to differentiated states, histone-regulated chromatin structure is crucial for maintaining developmental precision.
Cardiac and vascular tissues depend on histone H3 regulation during morphogenesis. Cardiomyocytes require coordinated chromatin remodeling to support chamber formation, contractile protein synthesis, and metabolic maturation. In endothelial tissues, histone H3-mediated transcriptional programs guide angiogenic sprouting, lumen formation, and vascular remodeling. Epigenetic changes mediated through H3 modifications contribute to developmental responses to hemodynamic forces and metabolic demands.
Endodermal organogenesis also involves substantial chromatin reorganization. Developing liver and pancreas undergo major transcriptional transitions that depend on histone H3 modifications governing metabolic, endocrine, and structural gene expression. Proper histone H3 dynamics ensure that hepatocytes and pancreatic progenitors respond appropriately to developmental cues that direct lineage specification and organ maturation.
Because histone H3 is central to nucleosome structure and epigenetic control, its detection is widely used in studies of chromatin biology, cell proliferation, lineage progression, and transcriptional regulation. This Zebrafish Histone H3 antibody is suitable for research involving developmental chromatin dynamics, neural and muscle differentiation, cardiac and vascular development, and epigenetic regulation across zebrafish tissues. NSJ Bioreagents provides this reagent within its zebrafish and chromatin-regulation antibody collection.
Optimal dilution of the Zebrafish Histone H3 antibody should be determined by the researcher.
An E.coli-derived zebrafish Histone H3 recombinant protein (amino acids Q56-R117) was used as the immunogen for the Zebrafish Histone H3 antibody.
After reconstitution, the Zebrafish Histone H3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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