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Home >> Antibodies >> Zebrafish Histone H3 Antibody

Zebrafish Histone H3 Antibody (RZ1070)

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  Catalog No Formulation Size Price (USD)  
Image RZ1070 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 539
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Western blot analysis of Histone H3 protein using Zebrafish Histone H3 antibody and 1) zebrafish head, 2) whole female zebrafish and 3) whole male zebrafish tissue lysate. Predicted molecular weight ~15 kDa.
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Western blot analysis of Histone H3 protein using Zebrafish Histone H3 antibody and 1) zebrafish head, 2) whole female zebrafish and 3) whole male zebrafish tissue lysate. Predicted molecular weight ~15 kDa.
Immunofluorescent analysis of Histone H3 protein using Zebrafish Histone H3 antibody (red) and zebrafish embryo tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE zebrafish brain tissue with Zebrafish Histone H3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE zebrafish liver tissue with Zebrafish Histone H3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE zebrafish colon tissue with Zebrafish Histone H3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE zebrafish muscle tissue with Zebrafish Histone H3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Availability 2-3 weeks
Species Reactivity Zebrafish
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity chromatography
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q6PI20
Applications Western Blot : 0.5-1 ug/ml
Immunohistochemistry (FFPE) : 2-5 ug/ml
Immunofluorescence : 5 ug/ml
Limitations This Zebrafish Histone H3 antibody is available for research use only.
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Description

Histone H3.1 is a protein that in humans is encoded by the HIST1H3A gene. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Application Notes

Optimal dilution of the Zebrafish Histone H3 antibody should be determined by the researcher.

Immunogen

An E.coli-derived zebrafish Histone H3 recombinant protein (amino acids Q56-R117) was used as the immunogen for the Zebrafish Histone H3 antibody.

Storage

After reconstitution, the Zebrafish Histone H3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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