Zebrafish HBBE3 Antibody / Embryonic Hemoglobin Beta Antibody (RZ1370)

Zebrafish HBBE3 Antibody Erythrocyte IHC. Immunohistochemistry analysis of HBBE3 using Zebrafish HBBE3 Antibody / Embryonic Hemoglobin Beta Antibody demonstrates strong cytoplasmic HRP-DAB brown staining within erythrocyte populations. The staining pattern is consistent with expression of Hemoglobin Beta Embryonic-3 (HBBE3), a beta-globin subunit involved in embryonic hemoglobin formation and oxygen transport during early zebrafish development. Robust staining of nucleated erythroid cells highlights the role of HBBE3 in primitive erythropoiesis and developing blood cell populations. The observed immunoreactivity supports the use of HBBE3 as a marker for embryonic erythrocytes, hematopoietic development, and studies of globin gene expression in zebrafish models. Heat-mediated antigen retrieval was performed in EDTA buffer prior to staining.

Zebrafish HBBE3 Antibody Western Blot. Western blot analysis of HBBE3 using Zebrafish HBBE3 Antibody / Embryonic Hemoglobin Beta Antibody demonstrates a distinct immunoreactive band at approximately 16 kDa in zebrafish head, whole female, and whole male tissue lysates. The observed molecular weight is consistent with the predicted size of Hemoglobin Beta Embryonic-3 (HBBE3), a beta-globin family member involved in embryonic hemoglobin formation and oxygen transport during early development. Comparable detection across the tested samples supports recognition of HBBE3 in zebrafish tissues and confirms antibody specificity for the target protein. The observed immunoreactivity demonstrates the utility of this antibody for studies of erythropoiesis, globin gene expression, hematopoietic development, and embryonic oxygen transport pathways in zebrafish models.