- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Zebrafish Gnaq Antibody / Guanine nucleotide-binding protein G(q) subunit alpha (RZ1128)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RZ1128 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 539 |
| Availability | 2-3 weeks |
| Species Reactivity | Zebrafish |
| Format | Antigen affinity purified |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit Ig |
| Purity | Antigen affinity chromatography |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| UniProt | B8JLJ7 |
| Localization | Cytoplasm |
| Applications | Western Blot : 0.5-1 ug/ml Immunohistochemistry (FFPE) : 2-5 ug/ml |
| Limitations | This Zebrafish Gnaq antibody is available for research use only. |
|
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Zebrafish (Danio rerio) Gnaq antibody detects Gnaq, a G protein alpha subunit that couples cell-surface receptors to intracellular signaling pathways controlling development, physiology, and cellular responsiveness. In zebrafish, the gnaq gene encodes Guanine nucleotide-binding protein G(q) subunit alpha, a member of the heterotrimeric G protein family that activates phospholipase C beta (PLC-beta) upon receptor stimulation. This activation initiates hydrolysis of phosphatidylinositol 4,5-bisphosphate into IP3 and diacylglycerol, driving calcium release from intracellular stores and activating protein kinase C. Because Gnaq integrates numerous extracellular cues into coordinated signaling responses, Zebrafish Gnaq antibody reagents are widely used in studies of signal transduction, neural development, and vertebrate physiology.
During zebrafish embryogenesis, gnaq expression is enriched in neural tissues, sensory structures, the heart, and developing musculature, reflecting its involvement in pathways regulating excitability, contraction, and calcium signaling. Gnaq-dependent signaling contributes to early patterning events by modulating responses to developmental cues such as growth factors, neurotransmitters, and chemokines. Disruption of Gq signaling in vertebrate systems affects cardiovascular function, pigmentation, sensory perception, and synaptic activity, underscoring the conserved role of Gnaq in organogenesis and homeostasis.
At the molecular level, Gnaq cycles between inactive GDP-bound and active GTP-bound states. Upon receptor activation, Gnaq exchanges GDP for GTP and engages downstream effectors including PLC-beta, TRPC channels, and regulators of calcium homeostasis. These interactions shape second-messenger signaling platforms that influence gene expression, cytoskeletal remodeling, and cellular metabolism. In zebrafish, Gnaq-driven calcium oscillations are important for muscle differentiation, neuronal circuit maturation, and coordinated tissue morphogenesis.
Gnaq also contributes to sensory and neurological processes. In vertebrates, Gq signaling is essential for phototransduction, taste transduction, and certain neuromodulatory pathways. Zebrafish exhibit comparable Gq-dependent processes, particularly in retinal development, neural crest-derived sensory organs, and hindbrain circuitry. These pathways rely on precise timing and magnitude of calcium signals that Gnaq helps regulate. Altered Gnaq activity can modify neuronal firing patterns, vascular tone, or heart rate, offering valuable insights into GPCR signaling behavior in vivo.
In addition to PLC-beta activation, Gnaq influences cytoskeletal dynamics by modifying Rho-family GTPase activity and interacting with scaffolding proteins that shape actin architecture. These functions support migration of neural crest cells, epithelial sheet movements, and cardiac morphogenesis. Zebrafish provide an excellent live-imaging system for visualizing these processes during development, enabling detailed analysis of Gnaq-regulated signaling networks.
Subcellular localization of Gnaq is primarily at the plasma membrane, where it interacts with G protein-coupled receptors (GPCRs) and downstream effectors. Active and inactive forms redistribute depending on receptor signaling status, allowing dynamic regulation of calcium-based signaling. Conservation of structural motifs and signaling behavior across vertebrates makes zebrafish a strong comparative model for studying Gq-pathway function in physiology and disease.
A Zebrafish Gnaq antibody is suitable for research applications such as western blotting, immunohistochemistry, and assays examining GPCR signaling, calcium mobilization, neural circuit development, and cardiovascular regulation. This antibody targets Gnaq for studies involving second-messenger signaling, receptor-mediated pathways, and vertebrate developmental biology. NSJ Bioreagents provides the Zebrafish Gnaq antibody to support research in intracellular signaling and physiological regulation.
Optimal dilution of the Zebrafish Gnaq antibody should be determined by the researcher.
An E.coli-derived zebrafish Gnaq recombinant protein (amino acids K102-D138) was used as the immunogen for the Zebrafish Gnaq antibody.
After reconstitution, the Zebrafish Gnaq antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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