Zebrafish GCLC Antibody / Glutamate-Cysteine Ligase Antibody (RZ1375)

Zebrafish GCLC Antibody WB. Western blot analysis of GCLC using Zebrafish GCLC Antibody / Glutamate-Cysteine Ligase Antibody demonstrates a prominent immunoreactive band at approximately 80 kDa in zebrafish head, whole female, whole male, and embryo tissue lysates. The observed molecular weight is slightly higher than the predicted size of approximately 73 kDa, which may reflect post-translational modification or differences in electrophoretic migration. GCLC is the catalytic subunit of glutamate-cysteine ligase, the rate-limiting enzyme in glutathione biosynthesis and a central regulator of cellular antioxidant defense pathways. Consistent detection across developmental and adult tissue samples supports broad expression of GCLC and confirms recognition of the target protein. The observed immunoreactivity demonstrates the utility of this antibody for studies of glutathione metabolism, oxidative stress responses, redox regulation, detoxification pathways, developmental biology, and cellular antioxidant defense mechanisms in zebrafish models.