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Home >> Antibodies >> Zebrafish Gbf1 Antibody / Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1

Zebrafish Gbf1 Antibody / Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 (RZ1114)

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Image RZ1114 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 539
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Immunofluorescent staining of FFPE zebrafish embryo tissue with Zebrafish Gbf1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
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Immunofluorescent staining of FFPE zebrafish embryo tissue with Zebrafish Gbf1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Western blot analysis of Gbf1 protein using Zebrafish Gbf1 antibody and 1) zebrafish head, 2) whole female zebrafish, 3) whole male zebrafish and 4) zebrafish embryo tissue lysate. Predicted molecular weight ~208 kDa.
IHC staining of FFPE zebrafish heart tissue with Zebrafish Gbf1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE zebrafish kidney tissue with Zebrafish Gbf1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE zebrafish brain tissue with Zebrafish Gbf1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Availability 2-3 weeks
Species Reactivity Zebrafish
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity chromatography
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt A0A286Y9X1
Localization Cytoplasm
Applications Western Blot : 0.5-1 ug/ml
Immunohistochemistry (FFPE) : 2-5 ug/ml
Immunofluorescence : 5 ug/ml
Limitations This Zebrafish Gbf1 antibody is available for research use only.
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Description

Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 is a protein that in humans is encoded by the GBF1 gene. This gene encodes a member of the Sec7 domain family. The encoded protein is a guanine nucleotide exchange factor that regulates the recruitment of proteins to membranes by mediating GDP to GTP exchange. The encoded protein is localized to the Golgi apparatus and plays a role in vesicular trafficking by activating ADP ribosylation factor 1. The encoded protein has also been identified as an important host factor for viral replication. Multiple transcript variants have been observed for this gene.

Application Notes

Optimal dilution of the Zebrafish Gbf1 antibody should be determined by the researcher.

Immunogen

An E.coli-derived zebrafish Gbf1 recombinant protein (amino acids E110-K1605) was used as the immunogen for the Zebrafish Gbf1 antibody.

Storage

After reconstitution, the Zebrafish Gbf1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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