Zebrafish GAD65 Antibody / GAD2 Antibody (RZ1356)

Zebrafish GAD65 / GAD2 Antibody Eye IHC. Immunohistochemistry staining of FFPE zebrafish eye tissue using Zebrafish GAD65 Antibody demonstrates prominent HRP-DAB brown staining within retinal cell layers associated with neuronal signaling and visual processing. The staining pattern is consistent with expression of Glutamate Decarboxylase 2 (GAD2), also known as GAD65, a key enzyme responsible for synthesis of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA). Strong retinal staining is consistent with the established role of GABAergic neurons in regulating visual signal transmission, retinal circuitry, and inhibitory neurotransmission within the vertebrate eye. Heat mediated antigen retrieval was performed in EDTA buffer. Primary antibody was incubated overnight at 4°C followed by detection using a peroxidase-conjugated goat anti-rabbit secondary antibody and DAB chromogen.

Zebrafish GAD65 / GAD2 Antibody Neurotransmitter Synthesis WB. Western blot analysis of zebrafish head tissue, whole female zebrafish, and whole male zebrafish lysates using Zebrafish GAD65 Antibody demonstrates a prominent immunoreactive band at approximately 65 kDa, consistent with the predicted molecular weight of Glutamate Decarboxylase 2 (GAD2), also known as GAD65. GAD65 is a key enzyme responsible for the synthesis of gamma-aminobutyric acid (GABA), the principal inhibitory neurotransmitter in the vertebrate nervous system. The observed band is detected across multiple zebrafish tissue preparations, consistent with the widespread importance of GABAergic signaling in neural function and neurotransmitter regulation. Minor lower-intensity bands are also present and may represent antibody-reactive species, alternatively processed forms, or non-specific background reactivity. Electrophoresis was performed on a 10% SDS-PAGE gel under reducing conditions followed by transfer to a nitrocellulose membrane. Signal was detected using an HRP-conjugated secondary antibody and enhanced chemiluminescent substrate.