Zebrafish FOXA1 Antibody / Forkhead Box A1 Antibody (RZ1379)

Zebrafish FOXA1 Antibody Embryo IF. Immunofluorescent staining of paraffin-embedded zebrafish embryo tissue using Zebrafish FOXA1 Antibody / Forkhead Box A1 Antibody demonstrates strong green fluorescence throughout developing embryonic tissue structures. The staining pattern is consistent with expression of FOXA1, a forkhead family transcription factor that functions as a pioneer regulator of chromatin accessibility and developmental gene expression. FOXA1 plays important roles in embryonic patterning, endoderm development, organogenesis, and tissue-specific differentiation. The observed staining highlights regions of active developmental regulation within the embryo, supporting the importance of FOXA1 during early tissue formation and maturation. Heat-mediated antigen retrieval was performed in EDTA buffer. Sections were blocked with 10% goat serum and incubated overnight at 4°C with 5 µg/ml primary antibody, followed by DyLight 488-conjugated goat anti-rabbit IgG secondary antibody at 1:500 dilution for 30 minutes at 37°C.

Zebrafish FOXA1 Antibody Western Blot. Western blot analysis of zebrafish head tissue lysate (lane 1), whole female zebrafish tissue lysate (lane 2), whole male zebrafish tissue lysate (lane 3), and zebrafish embryo tissue lysate (lane 4) using Zebrafish FOXA1 Antibody / Forkhead Box A1 Antibody demonstrates a distinct band at approximately 46 kDa, consistent with the expected molecular weight of FOXA1. FOXA1 is a forkhead family transcription factor and pioneer regulator that facilitates chromatin accessibility and controls gene expression programs involved in embryonic development, organogenesis, endoderm specification, and tissue differentiation. Detection of the expected molecular weight band in both adult and embryonic zebrafish samples supports the broad developmental role of FOXA1 and its utility as a marker of transcriptional regulation during tissue formation and maturation. Electrophoresis was performed using a 10% SDS-PAGE gel under reducing conditions with 30 µg of protein loaded per lane. Signal was visualized using HRP-conjugated secondary antibody and ECL detection.