Zebrafish FABP6 Antibody / Ileal Lipid Binding Protein Antibody (RZ1453)

Zebrafish FABP6 Antibody Brain IHC. Immunohistochemical analysis of FABP6 expression using anti-FABP6 antibody in paraffin embedded zebrafish brain tissue. FABP6, also known as Ileal Lipid Binding Protein and Gastrotropin, is a member of the fatty acid binding protein family that mediates intracellular transport of bile acids and other hydrophobic molecules involved in lipid metabolism. Distinct cytoplasmic staining is observed in neuronal and surrounding cells, suggesting expression of FABP6 in the zebrafish nervous system and supporting emerging roles for bile acid signaling in neural physiology. Heat mediated antigen retrieval was performed in EDTA buffer prior to staining. Sections were blocked with 10 percent goat serum and incubated with FABP6 antibody at 2 ug/ml overnight at 4 degrees Celsius. Detection was achieved using a peroxidase conjugated goat anti rabbit IgG secondary antibody and DAB chromogen. These results support the utility of Zebrafish FABP6 Antibody for studies of lipid metabolism, bile acid signaling, and developmental biology.

Zebrafish FABP6 Antibody WB. Western blot analysis of FABP6 expression using anti-FABP6 antibody. FABP6, also known as Ileal Lipid Binding Protein and Gastrotropin, is a member of the fatty acid binding protein family that mediates intracellular transport of bile acids and contributes to lipid homeostasis. Lane 1: whole female zebrafish tissue lysate. Lane 2: whole male zebrafish tissue lysate. A prominent band was detected at approximately 14 kDa, corresponding to the expected molecular weight of FABP6. Several faint higher molecular weight bands are also visible and may represent oligomeric species, post translationally modified forms, or nonspecific low abundance proteins. These results demonstrate expression of FABP6 in adult zebrafish tissues and support the utility of this antibody for studies of bile acid transport, lipid metabolism, and gastrointestinal physiology.