Zebrafish EpCAM Antibody / CD326 Antibody (RZ1369)

Zebrafish EpCAM Antibody Liver IHC. Immunohistochemistry staining of paraffin-embedded zebrafish liver tissue using Zebrafish EpCAM Antibody / CD326 Antibody demonstrates membranous and cytoplasmic HRP-DAB brown staining within hepatocyte populations and associated epithelial cell compartments. The staining pattern is consistent with expression of Epithelial Cell Adhesion Molecule (EPCAM/CD326), a transmembrane glycoprotein involved in epithelial cell adhesion, tissue organization, and regulation of cellular signaling pathways. Hepatic expression of EpCAM is consistent with its role in maintaining epithelial architecture and supporting cellular communication within organized tissue structures. The observed staining supports the use of EPCAM as a marker for studies investigating epithelial biology, tissue development, regenerative responses, and cell adhesion mechanisms in zebrafish. Heat-mediated antigen retrieval was performed in EDTA buffer prior to staining.

Zebrafish EpCAM Antibody Esophagus IHC. Immunohistochemistry staining of paraffin-embedded zebrafish esophagus tissue using Zebrafish EpCAM Antibody / CD326 Antibody demonstrates strong membranous HRP-DAB brown staining along the epithelial cell lining of the esophageal mucosa. The staining pattern is consistent with expression of Epithelial Cell Adhesion Molecule (EPCAM/CD326), a transmembrane glycoprotein that mediates epithelial cell-cell adhesion and contributes to maintenance of tissue architecture and epithelial integrity. Prominent localization at epithelial cell borders is characteristic of EpCAM expression and highlights its role in regulating cellular interactions within organized epithelial tissues. The observed staining supports the use of EPCAM as a marker for studies of epithelial differentiation, tissue organization, developmental biology, and regenerative processes in zebrafish. Heat-mediated antigen retrieval was performed in EDTA buffer prior to staining.

Zebrafish EpCAM Antibody Kidney IHC. Immunohistochemistry staining of paraffin-embedded zebrafish kidney tissue using Zebrafish EpCAM Antibody / CD326 Antibody demonstrates prominent membranous and cytoplasmic HRP-DAB brown staining within epithelial cells lining renal tubules and nephric structures. The staining pattern is consistent with expression of Epithelial Cell Adhesion Molecule (EPCAM/CD326), a transmembrane glycoprotein involved in epithelial cell adhesion, tissue organization, and maintenance of epithelial integrity. Renal epithelial localization is consistent with the established role of EpCAM in supporting cell-cell interactions and preserving the structural architecture of epithelial tissues. The observed staining highlights tubular epithelial compartments and supports the use of EPCAM as a marker for studies of epithelial biology, organ development, tissue homeostasis, and regenerative processes in zebrafish. Heat-mediated antigen retrieval was performed in EDTA buffer prior to staining.

Zebrafish EpCAM Antibody WB. Western blot analysis of zebrafish head, whole female, whole male, and embryo tissue lysates using Zebrafish EpCAM Antibody / CD326 Antibody demonstrates a prominent immunoreactive band at approximately 40 kDa in all tested samples. The observed molecular weight is slightly higher than the predicted size of Epithelial Cell Adhesion Molecule (EPCAM/CD326), consistent with post-translational modification of this transmembrane glycoprotein. Additional lower molecular weight immunoreactive bands are observed around ~25-30 kDa and may represent processed EPCAM fragments, degradation products, or other antibody-reactive species. EPCAM functions in epithelial cell adhesion, tissue organization, epithelial integrity, cellular signaling, and developmental processes.