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Home >> Antibodies >> Zebrafish Eno1 Antibody / Eno1a / Eno1b

Zebrafish Eno1 Antibody / Eno1a / Eno1b (RZ1132)

  Catalog No Formulation Size Price (USD)  
Image RZ1132 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 539
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IHC staining of FFPE zebrafish kidney tissue with Zebrafish Eno1 antibody, HRP secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot analysis of ENO1a/b protein using Zebrafish Eno1 antibody and 1) zebrafish head, 2) whole female zebrafish, 3) whole male zebrafish and 4) zebrafish embryo tissue lysate. Predicted molecular weight ~47 kDa.
Availability 2-3 weeks
Species Reactivity Zebrafish
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity chromatography
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q6PC89 , A0A2R8Q1X2
Localization Cytoplasm
Applications Western Blot : 0.5-1 ug/ml
Immunohistochemistry (FFPE) : 2-5 ug/ml
Limitations This Zebrafish Eno1 antibody is available for research use only.
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Description

This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy.

Application Notes

Optimal dilution of the Zebrafish Eno1 antibody should be determined by the researcher.

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of zebrafish ENO1a/b was used as the immunogen for the Zebrafish Eno1 antibody. This antibody will detect the a and b isoforms.

Storage

After reconstitution, the Zebrafish Eno1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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