Zebrafish DUSP6 Antibody / Dual Specificity Phosphatase 6 Antibody (RZ1384)

Zebrafish DUSP6 Antibody Embryo IF. Immunofluorescent staining of paraffin-embedded zebrafish embryo tissue using Zebrafish DUSP6 Antibody / Dual Specificity Phosphatase 6 Antibody demonstrates widespread green fluorescence within developing embryonic structures. The staining pattern is consistent with expression of DUSP6, a dual-specificity phosphatase that functions as a key negative regulator of ERK/MAPK signaling pathways. DUSP6 plays an important role in controlling growth factor-mediated signaling responses during embryogenesis, helping regulate cellular differentiation, tissue patterning, and organ development. The observed expression is consistent with the established involvement of DUSP6 in developmental signaling networks and feedback regulation of MAPK pathway activity. Nuclei are counterstained with DAPI (blue). Heat-mediated antigen retrieval was performed in EDTA buffer, and sections were incubated with 5 µg/ml primary antibody overnight at 4°C followed by DyLight-488 conjugated goat anti-rabbit IgG secondary antibody.

Zebrafish DUSP6 Antibody Brain IHC. Immunohistochemical staining of paraffin-embedded zebrafish brain tissue using Zebrafish DUSP6 Antibody / Dual Specificity Phosphatase 6 Antibody demonstrates widespread cytoplasmic HRP-DAB brown staining throughout neural tissue. The staining pattern is consistent with expression of DUSP6, a dual-specificity phosphatase that functions as a key negative regulator of ERK/MAPK signaling pathways. DUSP6 modulates growth factor-dependent signaling responses by dephosphorylating activated ERK proteins, thereby helping control signaling intensity, cellular differentiation, and tissue homeostasis. The observed expression within neural tissue is consistent with the importance of regulated MAPK signaling during nervous system development and maintenance. Heat-mediated antigen retrieval was performed in EDTA buffer. Sections were blocked with 10% goat serum and incubated overnight at 4°C with 2 µg/ml primary antibody, followed by peroxidase-conjugated goat anti-rabbit IgG for 30 minutes at 37°C. DAB was used as the chromogenic substrate.

Zebrafish DUSP6 Antibody WB. Western blot analysis of zebrafish head tissue lysate (lane 1) and zebrafish embryo tissue lysate (lane 2) using Zebrafish DUSP6 Antibody / Dual Specificity Protein Phosphatase 6 Antibody demonstrates a prominent immunoreactive band at approximately 42-45 kDa, consistent with the predicted molecular weight of DUSP6. DUSP6, also known as MKP-3, is a dual-specificity phosphatase that negatively regulates MAPK/ERK signaling and plays important roles in embryonic development, cell differentiation, tissue patterning, and growth factor-mediated signaling pathways. DUSP6 expression is widely studied in zebrafish developmental biology due to its involvement in FGF and ERK pathway regulation. Thirty micrograms of protein lysate were resolved under reducing conditions on a 10% SDS-PAGE gel and transferred to nitrocellulose prior to immunodetection. The observed bands support detection of zebrafish DUSP6 by western blot analysis.