Zebrafish DTNB Antibody / Dystrobrevin Alpha Antibody (RZ1411)

Zebrafish DTNB Antibody Spinal Cord IHC. Immunohistochemical analysis of paraffin-embedded zebrafish spinal cord tissue using Zebrafish DTNB Antibody / Dystrobrevin Alpha Antibody demonstrates strong cytoplasmic and fiber-associated HRP-DAB brown staining within elongated neural and supporting tissue structures. The staining pattern is consistent with expression of Dystrobrevin Alpha (DTNBA), a component of the dystrophin-associated protein complex involved in cytoskeletal organization, membrane stability, and neuromuscular signaling. Positive immunoreactivity highlights structures associated with spinal cord architecture and neuronal connectivity, supporting the role of DTNBA in nervous system development and maintenance. Heat-induced antigen retrieval was performed in EDTA buffer. Tissue sections were blocked with 10% goat serum and incubated with DTNBA antibody (2 ug/ml) overnight at 4°C, followed by Peroxidase Conjugated Goat Anti-rabbit IgG and DAB chromogen detection.

Zebrafish DTNB Antibody Zebrafish Tissue WB. Western blot analysis of 1) zebrafish head tissue lysate, 2) whole female zebrafish tissue lysate, and 3) whole male zebrafish tissue lysate using Zebrafish DTNB Antibody / Dystrobrevin Alpha Antibody. A prominent band is detected at approximately 66 kDa, consistent with the expected molecular weight of DTNBA / Dystrobrevin Alpha. Additional lower-intensity bands are observed and may represent DTNBA isoforms, post-translationally modified species, partial degradation products, or background reactivity commonly seen in complex whole-tissue lysates. Electrophoresis was performed on a 10% SDS-PAGE gel under reducing conditions with 30 ug sample per lane. Membranes were blocked with 5% non-fat milk/TBS and incubated with rabbit anti-DTNBA antibody at 0.5 ug/ml overnight at 4°C, followed by goat anti-rabbit IgG-HRP secondary antibody and ECL detection.