Zebrafish DSE Antibody / Dermatan Sulfate Epimerase Antibody (RZ1449)

Zebrafish DSE Antibody WB. Western blot analysis of DSE expression using anti-DSE antibody. DSE, also known as Dermatan Sulfate Epimerase, is an extracellular matrix biosynthetic enzyme that catalyzes the conversion of glucuronic acid to iduronic acid during dermatan sulfate synthesis. Lane 1: whole female zebrafish tissue lysate. Lane 2: whole male zebrafish tissue lysate. A major band was detected at approximately 180 kDa, above the predicted molecular weight of 110 kDa. Additional bands between approximately 55 and 90 kDa were also observed and may represent proteolytic fragments, alternatively processed forms, or post translationally modified species of Dermatan Sulfate Epimerase. The slower migration of the major band may reflect extensive glycosylation or other post translational modifications. These results demonstrate expression of DSE in adult zebrafish tissues and support the utility of this antibody for studies of extracellular matrix biology, glycosaminoglycan metabolism, and connective tissue development.