Zebrafish DPYD Antibody / Dihydropyrimidine Dehydrogenase Antibody (RZ1454)

Zebrafish DPYD Antibody Eye IHC. Immunohistochemical analysis of DPYD expression using anti-DPYD antibody in paraffin embedded zebrafish eye tissue. DPYD, also known as Dihydropyrimidine Dehydrogenase, is the rate limiting enzyme of pyrimidine catabolism and plays a critical role in nucleotide homeostasis and fluoropyrimidine metabolism. Diffuse cytoplasmic staining is observed throughout retinal layers and surrounding ocular tissues, consistent with the metabolic demands associated with visual system development and maintenance. Heat mediated antigen retrieval was performed in EDTA buffer prior to staining. Sections were blocked with 10 percent goat serum and incubated with DPYD antibody at 2 ug/ml overnight at 4 degrees Celsius. Detection was achieved using a peroxidase conjugated goat anti rabbit IgG secondary antibody and DAB chromogen. These results support the utility of Zebrafish DPYD Antibody for studies of pyrimidine metabolism, ocular development, and cellular homeostasis.

Zebrafish DPYD Antibody WB. Western blot analysis of DPYD using anti-DPYD antibody. Electrophoresis was performed on a 10% SDS-PAGE gel for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DPYD antigen antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus WB. Western blotting Substrate with Tanon 5200 system. A specific band was detected for DPYD at approximately 111 kDa. The expected band size for DPYD is at 111 kDa.