Zebrafish DIO3 Antibody / Iodothyronine Deiodinase 3 Antibody (RZ1417)

Zebrafish DIO3 Antibody Liver IHC. Immunohistochemical analysis of paraffin-embedded zebrafish liver tissue using Zebrafish DIO3 Antibody / Iodothyronine Deiodinase 3 Antibody demonstrates widespread cytoplasmic HRP-DAB brown staining throughout hepatocyte populations. The staining pattern is consistent with expression of DIO3A, a thyroid hormone-inactivating enzyme that regulates local concentrations of triiodothyronine (T3) and thyroxine (T4) to maintain endocrine homeostasis. Positive immunoreactivity within hepatic tissue highlights the important role of DIO3A in thyroid hormone metabolism, developmental regulation, and control of tissue-specific endocrine signaling. Heat-induced antigen retrieval was performed in EDTA buffer. Tissue sections were blocked with 10% goat serum and incubated with DIO3A antibody (2 ug/ml) overnight at 4°C, followed by Peroxidase Conjugated Goat Anti-rabbit IgG and DAB chromogen detection.

Zebrafish DIO3 Antibody Brain IHC. Immunohistochemical analysis of paraffin-embedded zebrafish brain tissue using Zebrafish DIO3 Antibody / Iodothyronine Deiodinase 3 Antibody demonstrates localized HRP-DAB brown staining within neural tissue surrounding densely cellular brain regions. The staining pattern is consistent with expression of DIO3A, a thyroid hormone-inactivating enzyme that regulates local availability of triiodothyronine (T3) and thyroxine (T4) during vertebrate development. DIO3A-mediated control of thyroid hormone signaling is essential for proper neurodevelopment, neuronal differentiation, and maintenance of endocrine homeostasis. Positive immunoreactivity within brain tissue supports the role of DIO3A in regulating tissue-specific thyroid hormone activity and developmental signaling pathways in the nervous system. Heat-induced antigen retrieval was performed in EDTA buffer. Tissue sections were blocked with 10% goat serum and incubated with DIO3A antibody (2 ug/ml) overnight at 4°C, followed by Peroxidase Conjugated Goat Anti-rabbit IgG and DAB chromogen detection.