Zebrafish DAZL Antibody / Germline Development Protein Antibody (RZ1409)

Zebrafish DAZL Antibody Head and Embryo WB. Western blot analysis of zebrafish head (lane 1) and embryo (lane 2) tissue lysates using Zebrafish DAZL Antibody / Germline Development Protein Antibody demonstrates a distinct immunoreactive band at approximately 30-35 kDa, consistent with expression of DAZL. DAZL is a conserved RNA-binding protein that regulates germline specification, germ cell survival, and reproductive development through control of mRNA translation and post-transcriptional gene expression. The strong signal observed in embryonic tissue is consistent with the established role of DAZL during early germline development and primordial germ cell formation. Although the predicted molecular weight of DAZL is approximately 25 kDa, the protein is observed at approximately 30-35 kDa, a migration pattern that has been reported for DAZL family proteins and may reflect phosphorylation and anomalous electrophoretic mobility of this RNA-binding protein. Primary antibody was used at 0.5 ug/ml. Predicted molecular weight: approximately 25 kDa. Observed molecular weight: approximately 30-35 kDa.