Zebrafish CYP3A65 Antibody / Cytochrome P450 Enzyme Antibody (RZ1367)

Zebrafish CYP3A65 Antibody Liver IHC. Immunohistochemistry staining of paraffin-embedded zebrafish liver tissue using Zebrafish CYP3A65 Antibody / Cytochrome P450 Enzyme Antibody demonstrates predominantly cytoplasmic HRP-DAB brown staining throughout hepatocyte populations. The staining pattern is consistent with expression of Cytochrome P450 3A65 (CYP3A65), a member of the cytochrome P450 enzyme family involved in xenobiotic metabolism, detoxification, and oxidative biotransformation of endogenous and exogenous compounds. Hepatic localization is consistent with the established role of CYP3A family enzymes in drug metabolism and chemical processing within the liver. The observed staining highlights metabolically active hepatocytes and supports the use of CYP3A65 as a marker for studies of toxicology, pharmacology, metabolic regulation, and environmental response pathways in zebrafish. Heat-mediated antigen retrieval was performed in pH 8.0 EDTA buffer prior to staining.

Zebrafish CYP3A65 Antibody Brain IHC. Immunohistochemistry staining of paraffin-embedded zebrafish brain tissue using Zebrafish CYP3A65 Antibody / Cytochrome P450 Enzyme Antibody demonstrates cytoplasmic HRP-DAB brown staining within neuronal and neuropil-rich regions of the brain. The staining pattern is consistent with expression of Cytochrome P450 3A65 (CYP3A65), a member of the cytochrome P450 superfamily involved in oxidative metabolism of endogenous signaling molecules and xenobiotic compounds. Expression within neural tissue is consistent with the growing recognition that cytochrome P450 enzymes contribute to local metabolic regulation, neuroactive compound processing, and maintenance of biochemical homeostasis within the nervous system. The observed staining supports the use of CYP3A65 as a target for studies investigating xenobiotic metabolism, neurotoxicology, and metabolic regulation in zebrafish. Heat-mediated antigen retrieval was performed in EDTA buffer prior to staining.

Zebrafish CYP3A65 Antibody Eye IHC. Immunohistochemistry staining of paraffin-embedded zebrafish eye tissue using Zebrafish CYP3A65 Antibody / Cytochrome P450 Enzyme Antibody demonstrates strong cytoplasmic HRP-DAB brown staining within retinal cell layers, with prominent immunoreactivity extending across multiple neural and photoreceptor-associated regions of the retina. The staining pattern is consistent with expression of Cytochrome P450 3A65 (CYP3A65), a member of the cytochrome P450 enzyme family involved in xenobiotic metabolism, detoxification, and oxidative processing of endogenous signaling molecules. Retinal expression is consistent with a role for CYP3A65 in local metabolic regulation and protection of ocular tissues from endogenous and environmental compounds. The observed staining highlights the metabolically active nature of retinal cells and supports the use of CYP3A65 as a target for studies of ocular biology, toxicology, and cytochrome P450-mediated metabolic pathways in zebrafish. Heat-mediated antigen retrieval was performed in EDTA buffer prior to staining.

Zebrafish CYP3A65 Antibody Ovary IHC. Immunohistochemistry staining of paraffin-embedded zebrafish ovary tissue using Zebrafish CYP3A65 Antibody / Cytochrome P450 Enzyme Antibody demonstrates strong cytoplasmic and membranous HRP-DAB brown staining within ovarian follicular cells surrounding developing oocytes. The staining pattern is consistent with expression of Cytochrome P450 3A65 (CYP3A65), a member of the cytochrome P450 enzyme family involved in xenobiotic metabolism and oxidative processing of endogenous compounds. Ovarian localization is consistent with the role of cytochrome P450 enzymes in steroid metabolism, reproductive physiology, and regulation of hormone-associated signaling pathways. The prominent staining observed in follicle-associated cell populations highlights metabolically active compartments involved in oocyte development and reproductive function. These findings support the use of CYP3A65 as a target for studies of zebrafish reproductive biology, endocrine regulation, toxicology, and cytochrome P450-mediated metabolic pathways. Heat-mediated antigen retrieval was performed in EDTA buffer prior to staining.

Zebrafish CYP3A65 Antibody WB. Western blot analysis of zebrafish whole female and whole male tissue lysates using Zebrafish CYP3A65 Antibody / Cytochrome P450 Enzyme Antibody demonstrates a prominent immunoreactive band at approximately 59 kDa. The observed molecular weight is consistent with the predicted size of Cytochrome P450 3A65 (CYP3A65), a member of the cytochrome P450 superfamily involved in xenobiotic metabolism, detoxification, and oxidative biotransformation of endogenous and exogenous compounds. Detection of a distinct band in both female and male zebrafish samples supports expression of CYP3A65 across adult tissues and is consistent with its established role in metabolic processing and chemical homeostasis. CYP3A65 is frequently studied as a zebrafish model of CYP3A-mediated drug metabolism and toxicological response pathways.