Zebrafish Cyclin E1 Antibody / CCNE1 Antibody (RZ1362)

Zebrafish Cyclin E1 / CCNE1 Antibody Cell Cycle WB. Western blot analysis of zebrafish head tissue, whole female zebrafish, whole male zebrafish, and zebrafish embryo lysates using Zebrafish Cyclin E1 Antibody demonstrates a prominent immunoreactive band at approximately 46 kDa, consistent with the predicted molecular weight of Cyclin E1 (CCNE1). Cyclin E1 is a key regulator of G1/S cell cycle progression that functions through activation of CDK2 to promote DNA replication and cellular proliferation. The observed expression across multiple zebrafish tissue preparations, including embryonic tissue, is consistent with the established role of CCNE1 in developmental growth, tissue expansion, and regulation of cell cycle progression. Minor higher molecular weight immunoreactive bands are present in some samples and may represent post-translationally modified forms, protein complexes, or other antibody-reactive species. Electrophoresis was performed on a 10% SDS-PAGE gel under reducing conditions followed by transfer to a nitrocellulose membrane. Signal was detected using an HRP-conjugated secondary antibody and enhanced chemiluminescent substrate.