Zebrafish CXCR7A Antibody / ACKR3A Antibody (RZ1330)

Zebrafish CXCR7A Antibody Spinal Cord IHC. Immunohistochemistry staining of FFPE zebrafish spinal cord tissue using Zebrafish CXCR7A Antibody demonstrates diffuse cytoplasmic and membrane-associated HRP-DAB brown staining throughout elongated neural tissue structures. The staining pattern is consistent with expression of CXC Chemokine Receptor 7A (CXCR7A), also known as Atypical Chemokine Receptor 3A (ACKR3A), a chemokine receptor involved in regulation of CXCL12 signaling, cell migration, and developmental tissue patterning. Signal is observed within spinal cord-associated cellular compartments and neural processes, reflecting the established role of CXCR7A in directing cellular positioning and organization during vertebrate development. Heat mediated antigen retrieval was performed in EDTA buffer. Primary antibody was incubated overnight at 4°C followed by detection using a peroxidase-conjugated goat anti-rabbit secondary antibody and DAB chromogen.

Zebrafish CXCR7A Antibody Chemokine Receptor WB. Western blot analysis of zebrafish head tissue, whole female zebrafish, whole male zebrafish, and zebrafish embryo lysates using Zebrafish CXCR7A Antibody demonstrates a prominent immunoreactive band at approximately 40 kDa, consistent with the predicted molecular weight of CXC Chemokine Receptor 7A (CXCR7A). Also known as Atypical Chemokine Receptor 3A (ACKR3A), CXCR7A functions as an atypical chemokine receptor that regulates CXCL12 availability and contributes to cell migration, tissue patterning, and developmental signaling pathways. Comparable detection across multiple zebrafish tissue sources is consistent with the broad biological functions of CXCR7A during vertebrate development. Electrophoresis was performed on a 10% SDS-PAGE gel under reducing conditions followed by transfer to a nitrocellulose membrane. Signal was detected using an HRP-conjugated secondary antibody and enhanced chemiluminescent substrate. Predicted molecular weight: ~40 kDa.