Zebrafish COL10A1 Antibody / Collagen Type X Alpha 1a Antibody (RZ1430)

Zebrafish COL10A1 Antibody Gill IHC. Immunohistochemistry staining of paraffin-embedded zebrafish gill tissue using COL10A1 antibody demonstrates prominent staining within extracellular matrix-rich structural elements of the gill apparatus, consistent with expression of Collagen Type X Alpha 1a (COL10A1A). COL10A1A is a structural extracellular matrix protein that contributes to cartilage maturation, matrix organization, and skeletal development. The observed staining pattern within supportive gill structures is consistent with the established role of type X collagen family members in maintaining specialized extracellular matrix environments and supporting tissue architecture during vertebrate development. Heat-mediated antigen retrieval was performed in EDTA buffer. Tissue sections were stained with 2 ug/ml primary antibody and visualized using DAB chromogen.

Zebrafish COL10A1 Antibody IHC. Immunohistochemistry analysis of COL10A1A using anti-COL10A1 antibody. COL10A1A was detected in a paraffin-embedded section of zebrafish vertebra tissue. Heat mediated antigen retrieval was performed in EDTA buffer. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-COL10A1A Antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using DAB as the chromogen.

Zebrafish COL10A1 Antibody Tooth IHC. Immunohistochemistry staining of paraffin-embedded zebrafish tooth tissue using COL10A1 antibody demonstrates intense staining within mineralized tooth-associated matrix structures, consistent with expression of Collagen Type X Alpha 1a (COL10A1A). COL10A1A is a structural extracellular matrix protein that contributes to cartilage maturation, matrix organization, and skeletal development. The observed staining pattern within odontogenic and mineralized tissue compartments is consistent with the established role of type X collagen family members in formation and maintenance of specialized extracellular matrix environments associated with skeletal and craniofacial development. Heat-mediated antigen retrieval was performed in EDTA buffer. Tissue sections were stained with 2 ug/ml primary antibody and visualized using DAB chromogen.