Zebrafish Caspase 9 Antibody / Casp9 (RZ1208)

Description

Caspase 9 is a key initiator caspase in the intrinsic (mitochondrial) apoptotic pathway, responsible for activating downstream effector caspases and orchestrating programmed cell death. In zebrafish (Danio rerio), Caspase 9 plays a conserved role in regulating apoptosis during embryonic development, as well as in maintaining tissue homeostasis and responding to cellular stress.

Following mitochondrial outer membrane permeabilization, cytochrome c is released into the cytosol, leading to the formation of the apoptosome complex. Caspase 9 is recruited and activated within this complex, initiating a proteolytic cascade that culminates in the activation of effector caspases such as Caspase 3 and Caspase 7. In zebrafish, casp9 is expressed in multiple tissues during development and is crucial for eliminating damaged, mis-specified, or excess cells.

Zebrafish Caspase 9 is widely used in research on cell death mechanisms, neurodevelopment, cancer biology, and toxicology, providing an in vivo model to study mitochondria-mediated apoptosis and the effects of pro- and anti-apoptotic signals. Due to its high degree of functional conservation with mammalian caspase-9, it serves as a valuable tool for both basic and translational research.

Application Notes

Optimal dilution of the Zebrafish Caspase 9 antibody should be determined by the researcher.

Immunogen

E. coli-derived zebrafish Caspase 9 recombinant protein (amino acids K18-Q417) was used as the immunogen for the Zebrafish Caspase 9 antibody.

Storage

After reconstitution, the Zebrafish Caspase 9 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.