Zebrafish CDH17 Antibody / Cadherin-17 Antibody (RZ1399)

Zebrafish CDH17 Antibody IF. Immunofluorescent staining of paraffin-embedded zebrafish embryo tissue using Zebrafish CDH17 Antibody / Cadherin-17 Antibody demonstrates green fluorescence within developing epithelial and embryonic tissue compartments. The staining pattern is consistent with expression of CDH17 (Cadherin-17), a calcium-dependent cell adhesion molecule involved in epithelial organization, tissue integrity, and digestive tract development. CDH17 is widely used as a marker of intestinal epithelial differentiation and gastrointestinal organogenesis in zebrafish and other vertebrate model systems. HIER was performed using EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with primary antibody at 5 ug/ml overnight at 4C. DyLight-488-conjugated goat anti-rabbit IgG was used as the secondary antibody (green), and nuclei were counterstained with DAPI (blue). The image supports detection of CDH17 expression in developing zebrafish embryonic tissues by immunofluorescence.

Zebrafish CDH17 Antibody Colon IHC. Immunohistochemistry staining of paraffin-embedded zebrafish colon tissue using Zebrafish CDH17 Antibody / Cadherin-17 Antibody demonstrates strong membranous and cytoplasmic HRP-DAB brown staining within intestinal epithelial cells lining glandular and luminal structures. The staining pattern is consistent with expression of CDH17 (Cadherin-17), a calcium-dependent cell adhesion molecule involved in epithelial organization, maintenance of tissue integrity, and gastrointestinal tract development. CDH17 is widely recognized as a marker of intestinal epithelial differentiation and digestive system maturation in zebrafish. HIER was performed using EDTA buffer. Tissue sections were blocked with 10% goat serum and incubated with primary antibody at 2 ug/ml overnight at 4C prior to detection with peroxidase-conjugated goat anti-rabbit IgG and DAB chromogen.

Zebrafish CDH17 Antibody Kidney IHC. Immunohistochemistry staining of paraffin-embedded zebrafish kidney tissue using Zebrafish CDH17 Antibody / Cadherin-17 Antibody demonstrates strong membranous and apical HRP-DAB brown staining within epithelial cells lining renal tubular structures. The staining pattern is consistent with expression of CDH17 (Cadherin-17), a calcium-dependent cell adhesion molecule that contributes to epithelial organization, maintenance of tissue architecture, and regulation of cell-cell adhesion. CDH17 is widely studied as an epithelial marker and plays important roles in tissue differentiation and organ development. HIER was performed using EDTA buffer. Tissue sections were blocked with 10% goat serum and incubated with primary antibody at 2 ug/ml overnight at 4C prior to detection with peroxidase-conjugated goat anti-rabbit IgG and DAB chromogen.

Zebrafish CDH17 Antibody WB. Western blot analysis of zebrafish head tissue lysate (lane 1), whole female zebrafish tissue lysate (lane 2), and whole male zebrafish tissue lysate (lane 3) using Zebrafish CDH17 Antibody / Cadherin-17 Antibody demonstrates a prominent immunoreactive band at approximately 96-120 kDa, consistent with expression of CDH17. Cadherin-17 is a calcium-dependent cell adhesion molecule involved in epithelial organization, maintenance of tissue integrity, and regulation of cell-cell interactions within digestive and epithelial tissues. CDH17 is widely used as a marker of intestinal epithelial differentiation and gastrointestinal tract development in zebrafish. The observed molecular weight above the predicted size may reflect post-translational modification, including glycosylation, which is common among cadherin family proteins. Thirty micrograms of protein were loaded per lane under reducing conditions. Predicted molecular weight: approximately 96 kDa.