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Home >> Antibodies >> Zebrafish Atp6v1a Antibody

Zebrafish Atp6v1a Antibody (RZ1129)

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Image RZ1129 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 539
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Immunohistochemical analysis of Atp6v1a protein using Zebrafish Atp6v1a antibody and paraffin-embedded zebrafish spinal cord tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Immunohistochemical analysis of Atp6v1a protein using Zebrafish Atp6v1a antibody and paraffin-embedded zebrafish spinal cord tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot analysis of Atp6v1a protein using Zebrafish ATP6V1A antibody and zebrafish head tissue lysate. The predicted molecular weight of Atp6v1a is ~68 kDa.
Immunohistochemical analysis of Atp6v1a protein using Zebrafish Atp6v1a antibody and paraffin-embedded zebrafish brain tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunohistochemical analysis of Atp6v1a protein using Zebrafish Atp6v1a antibody and paraffin-embedded zebrafish kidney tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunohistochemical analysis of Atp6v1a protein using Zebrafish Atp6v1a antibody and paraffin-embedded zebrafish liver tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunohistochemical analysis of Atp6v1a protein using Zebrafish Atp6v1a antibody and paraffin-embedded zebrafish spleen tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunohistochemical analysis of Atp6v1a protein using Zebrafish Atp6v1a antibody and paraffin-embedded zebrafish intestinal tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Availability 2-3 weeks
Species Reactivity Zebrafish
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity chromatography
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt E7FCD8
Localization Plasma membrane
Applications Western Blot : 0.5-1 ug/ml
Immunohistochemistry (FFPE) : 2-5 ug/ml
Limitations This Zebrafish Atp6v1a antibody is available for research use only.
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Description

V-type proton ATPase catalytic subunit A is an enzyme that in humans is encoded by the ATP6V1A gene. This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c, and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist.

Application Notes

Optimal dilution of the Zebrafish Atp6v1a antibody should be determined by the researcher.

Immunogen

An E.coli-derived zebrafish Atp6v1a recombinant protein (amino acids K139-D617) was used as the immunogen for the Zebrafish Atp6v1a antibody.

Storage

After reconstitution, the Zebrafish Atp6v1a antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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