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Home >> Antibodies >> Zebrafish Atp5b Antibody / Atp5f1b / ATP synthase subunit beta

Zebrafish Atp5b Antibody / Atp5f1b / ATP synthase subunit beta (RZ1029)

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Image RZ1029 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 539
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IHC staining of FFPE zebrafish brain tissue with Zebrafish Atp5b antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE zebrafish brain tissue with Zebrafish Atp5b antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot analysis of Atp5b/ATP5F1B protein using Zebrafish Atp5b antibody and 1) zebrafish head 2) female zebrafish viscera, 3) male zebrafish viscera, 4) whole zebrafish and 5) zebrafish embryo tissue lysate. Predicted molecular weight ~57 kDa.
IHC staining of FFPE zebrafish colon tissue with Zebrafish Atp5b antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE zebrafish kidney tissue with Zebrafish Atp5b antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE zebrafish embryo tissue with Zebrafish Atp5b antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Availability 2-3 weeks
Species Reactivity Zebrafish
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity chromatography
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt A8WGC6
Applications Western Blot : 0.5-1 ug/ml
Immunohistochemistry (FFPE) : 2-5 ug/ml
Immunofluorescence : 5 ug/ml
Limitations This Zebrafish Atp5b antibody is available for research use only.
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Description

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core.

Application Notes

Optimal dilution of the Zebrafish Atp5b antibody should be determined by the researcher.

Immunogen

An E.coli-derived zebrafish Atp5b/ATP5F1B recombinant protein (amino acids Q112-S517) was used as the immunogen for the Zebrafish Atp5b antibody.

Storage

After reconstitution, the Zebrafish Atp5b antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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