Zebrafish PYCARD Antibody / ASC Antibody (RZ1474)

Zebrafish PYCARD Antibody / ASC Antibody Zebrafish Eye IHC. Immunohistochemical staining of paraffin-embedded zebrafish eye tissue using Zebrafish PYCARD Antibody / ASC Antibody. PYCARD expression is detected within retinal cell layers, consistent with its role as an inflammasome adaptor protein that mediates innate immune signaling, inflammatory responses, and programmed cell death. Heat-induced epitope retrieval was performed using EDTA buffer prior to immunostaining with DAB chromogenic detection.

Zebrafish PYCARD Antibody / ASC Antibody Zebrafish Head, Female, Male and Embryo WB. Western blot analysis of zebrafish head, whole female, whole male, and embryo tissue lysates using Zebrafish PYCARD Antibody / ASC Antibody. A distinct immunoreactive band is detected at approximately 23 kDa in all four samples, consistent with expression of PYCARD (ASC). The observed molecular weight matches the predicted size of approximately 23 kDa, supporting specific detection of the target protein. Proteins were separated under reducing conditions, transferred to a nitrocellulose membrane, and detected following incubation with the primary antibody (0.5 ug/ml) and an HRP-conjugated goat anti-rabbit secondary antibody using ECL detection.