Zebrafish ACACA Antibody / Acetyl-CoA Carboxylase 1 Antibody (RZ1336)

Zebrafish ACACA / Acetyl-CoA Carboxylase 1 Antibody Embryo IF. Immunofluorescent staining of FFPE zebrafish embryo tissue using Zebrafish ACACA Antibody demonstrates distinct green fluorescence throughout developing embryonic tissue compartments against a blue DAPI nuclear counterstain background. The staining pattern is consistent with expression of Acetyl-CoA Carboxylase 1 (ACACA), also known as ACC1, a rate-limiting enzyme in de novo fatty acid biosynthesis that catalyzes the conversion of acetyl-CoA to malonyl-CoA. Signal is observed within embryonic structures undergoing active growth and metabolic development, reflecting the essential role of ACACA in lipid synthesis, membrane biogenesis, and cellular energy homeostasis during vertebrate embryogenesis. Heat mediated antigen retrieval was performed in EDTA buffer. Primary antibody was incubated overnight at 4oC followed by detection using a DyLight 488-conjugated goat anti-rabbit secondary antibody. Nuclei were counterstained with DAPI.

Zebrafish ACACA / Acetyl-CoA Carboxylase 1 Antibody Ovary IHC. Immunohistochemistry staining of FFPE zebrafish ovary tissue using Zebrafish ACACA Antibody demonstrates cytoplasmic HRP-DAB brown staining within ovarian follicular and oocyte-associated cellular compartments. The staining pattern is consistent with expression of Acetyl-CoA Carboxylase 1 (ACACA), also known as ACC1, a rate-limiting enzyme in fatty acid biosynthesis that catalyzes the formation of malonyl-CoA from acetyl-CoA. Prominent staining within lipid-rich ovarian tissue is consistent with the essential role of ACACA in lipid metabolism, membrane biogenesis, energy storage, and reproductive tissue development. Heat mediated antigen retrieval was performed in EDTA buffer. Primary antibody was incubated overnight at 4°C followed by detection using a peroxidase-conjugated goat anti-rabbit secondary antibody and DAB chromogen.

Zebrafish ACACA / Acetyl-CoA Carboxylase 1 Antibody Liver IHC. Immunohistochemistry staining of FFPE zebrafish liver tissue using Zebrafish ACACA Antibody demonstrates granular cytoplasmic HRP-DAB brown staining throughout hepatocyte-associated cellular populations. The staining pattern is consistent with expression of Acetyl-CoA Carboxylase 1 (ACACA), also known as ACC1, a rate-limiting enzyme in de novo fatty acid biosynthesis that catalyzes the conversion of acetyl-CoA to malonyl-CoA. Prominent hepatic staining is consistent with the central role of ACACA in lipid metabolism, energy storage, and regulation of fatty acid synthesis within the liver. Heat mediated antigen retrieval was performed in EDTA buffer. Primary antibody was incubated overnight at 4°C followed by detection using a peroxidase-conjugated goat anti-rabbit secondary antibody and DAB chromogen.

Zebrafish ACACA / Acetyl-CoA Carboxylase 1 Antibody Lipid Metabolism WB. Western blot analysis of zebrafish head tissue lysate using Zebrafish ACACA Antibody demonstrates a prominent immunoreactive band at approximately 269 kDa, consistent with the predicted molecular weight of Acetyl-CoA Carboxylase 1 (ACACA), also known as ACC1. ACACA is a biotin-dependent enzyme that catalyzes the conversion of acetyl-CoA to malonyl-CoA, the committed and rate-limiting step in de novo fatty acid biosynthesis. The observed band is consistent with expression of this key metabolic regulator, which plays an essential role in lipid synthesis, energy storage, membrane biogenesis, and cellular metabolic homeostasis. Electrophoresis was performed on an 8% SDS-PAGE gel under reducing conditions followed by transfer to a nitrocellulose membrane. Signal was detected using an HRP-conjugated secondary antibody and enhanced chemiluminescent substrate. Predicted molecular weight: ~269 kDa. Observed molecular weight: ~269 kDa.