ZC3H7A Antibody / RNA Processing Factor Antibody [clone PCRP-ZC3H7A-1D6] (V9472)

ZC3H7A Antibody HeLa IF. Immunofluorescence analysis of PFA-fixed human HeLa cells using ZC3H7A Antibody / RNA Processing Factor Antibody clone PCRP-ZC3H7A-1D6 demonstrates prominent green fluorescence throughout the cytoplasm with additional signal extending into the perinuclear region, consistent with the reported role of Zinc finger CCCH-type containing 7A (ZC3H7A) as an RNA-binding protein involved in RNA metabolism and post-transcriptional gene regulation. The staining pattern highlights the intracellular distribution expected for a protein associated with RNA processing complexes and messenger RNA regulatory pathways. Cellular architecture is visualized using phalloidin (red), which labels filamentous actin and outlines cell morphology. This immunofluorescence staining pattern supports studies of RNA processing factor biology, RNA-binding protein function, and post-transcriptional regulatory mechanisms in human cells. ZC3H7A antibody is shown in green and phalloidin is shown in red.

ZC3H7A Antibody HeLa FACS. Flow cytometry analysis of PFA-fixed human HeLa cells using ZC3H7A Antibody / RNA Processing Factor Antibody clone PCRP-ZC3H7A-1D6 demonstrates a distinct rightward shift of the blue histogram relative to the isotype control (red), indicating specific detection of Zinc finger CCCH-type containing 7A (ZC3H7A) expression. ZC3H7A is a CCCH-type zinc finger RNA-binding protein involved in RNA metabolism, messenger RNA processing, and post-transcriptional regulation of gene expression. The observed fluorescence shift is consistent with expression of this RNA processing factor in human HeLa cells and supports the use of clone PCRP-ZC3H7A-1D6 for studies of RNA-binding protein biology, transcript regulation, and cellular gene expression pathways. Blue = ZC3H7A antibody; Red = isotype control.

SDS-PAGE analysis of purified, BSA-free ZC3H7A antibody (clone PCRP-ZC3H7A-1D6) as confirmation of integrity and purity.

ZC3H7A Antibody Protein Microarray Validation. Analysis of a HuProt(TM) protein microarray containing more than 19,000 full-length human proteins using ZC3H7A Antibody / RNA Processing Factor Antibody clone PCRP-ZC3H7A-1D6 demonstrates exceptional target specificity for Zinc finger CCCH-type containing 7A (ZC3H7A). The intended target produced the highest signal on the array with a Z-score of 107.56 and an S-score of 92.42, greatly exceeding all other detected proteins. The next highest signals were observed for RTN2 and FAF1 with substantially lower Z-scores and S-scores, supporting highly selective target recognition by clone PCRP-ZC3H7A-1D6. ZC3H7A is a CCCH-type zinc finger RNA-binding protein involved in RNA metabolism and post-transcriptional gene regulation. The strong separation between the intended target and all other proteins on the array supports the use of this RNA Processing Factor Antibody for studies of RNA-binding protein biology and messenger RNA regulatory pathways. The Z-score represents signal strength relative to the array mean, while the S-score represents the specificity gap between successive ranked targets. An S-score greater than 2.5 is generally considered indicative of high target specificity.