ZAP70 Antibody for FACS / Lymphocyte Activation Flow Cytometry Antibody [clone 9D5] (RQ5941)

ZAP70 Antibody for FACS / Lymphocyte Activation Flow Cytometry Antibody. Flow cytometry analysis of human Jurkat T lymphocyte cells evaluating intracellular ZAP70 expression associated with lymphocyte activation using clone ZAP70/2047 at 1 ug per million cells. Cells were fixed, permeabilized, and blocked with goat sera prior to staining to support accurate detection of the cytoplasmic kinase Zeta-chain-associated protein kinase 70 (ZAP70), a marker linked to activated T-cell signaling states. The blue histogram shows a pronounced rightward shift corresponding to ZAP70-positive activated lymphocyte populations, the green histogram represents the isotype control, and the red histogram indicates unstained cells, confirming activation-associated intracellular ZAP70 detection by flow cytometry.

ZAP70 Antibody for FACS / Lymphocyte Activation Flow Cytometry Antibody. Flow cytometry analysis of human 293T cells assessing intracellular ZAP70 expression using clone ZAP70/2047 at 1 ug per million cells. Cells were fixed, permeabilized, and blocked with goat sera prior to staining to enable detection of the cytoplasmic kinase Zeta-chain-associated protein kinase 70 (ZAP70), supporting evaluation of activation-associated signaling features outside of classical lymphocyte models. The blue histogram shows a rightward fluorescence shift corresponding to ZAP70 antibody staining, the green histogram represents the isotype control, and the red histogram indicates unstained cells, demonstrating specific intracellular detection of ZAP70 by flow cytometry.

Western blot testing of 1) human Jurkat, 2) human CCRF-CEM, 3) rat thymus, 4) mouse spleen and 5) mouse thymus lysate with ZAP70 antibody. Expected molecular weight ~70 kDa.